LOCUS       sequence-86466-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E.coli expression of GST tagged human RanBP2 fragment with SUMO 
            ligase activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Flotho A, Werner A, Winter T, Frank AS, Ehret H, Melchior F
  TITLE     Recombinant reconstitution of sumoylation reactions in vitro.
  JOURNAL   Methods Mol Biol. 2012;832:93-110. doi: 10.1007/978-1-61779-474-2_5.
  PUBMED    22350878
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2012"; volume: "832:93-110. doi"; pages: " 
            10.1007/978-1-61779-474-2_5"
COMMENT     Sequence Label: pGEX3x-hRanBP2-(IR1+M)
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..14
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(709..731)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tgatcgaagg tcgtgggatc cccgatgttc tcattgtata tgaactaact ccaaccgctg
       61 agcagaaagc ccttgcaacc aaacttaaac ttcctccaac tttcttctgc tacaagaata
      121 gaccagatta tgttagtgaa gaagaggagg atgatgaaga tttcgaaaca gctgtcaaga
      181 aacttaatgg aaaactatat ttggatggct cagaaaaatg tagacccttg gaagaaaata
      241 cagcagataa tgagaaagaa tgtatttgag tttgggaaaa gaaaccaaca gttgaagaga
      301 aggcaaaagc agatacgtta aaacttccac ctacattttt ttgtggagtc tgtagtgata
      361 ctgatgaaga caatggaaat ggggaagact ttcaatcaga gcttcaaaaa gttcaggaag
      421 ctcaaaaatc tcagacagaa gaaataacta gcacaactga cagtgtatat acaggtggga
      481 ctgaagtgat ggtaccttct ttctgtaaat ctgaagaacc tgattctatt accaaatcca
      541 ttagttcacc atctgtttcc tctgaaacta tggacaaacc tgtagatttg tcaactagaa
      601 aggaaattga tacagattct acaagccaag gggaaagcaa gatagtttag gaattcatcg
      661 tgactgactg acgatctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
      721 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
      781 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
      841 cgatagc
//
