LOCUS       sequence-86470-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E.coli expression of full length untagged mouse Ubc9 (protein 
            sequence identical to human).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Pichler A, Gast A, Seeler JS, Dejean A, Melchior F
  TITLE     The nucleoporin RanBP2 has SUMO1 E3 ligase activity.
  JOURNAL   Cell. 2002 Jan 11;108(1):109-20.
  PUBMED    11792325
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2002-01-11"; volume: "108(1)"; pages: "109-20"
COMMENT     Sequence Label: pET23a-mUbc9
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             555..572
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(625..643)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      639..686
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(810..829)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgtcgggg atcgccctca gccgccttgc
       61 gcaggaaagg aaagcctgga ggaaggacca cccttttggc tttgtagctg tcccaacaaa
      121 gaaccctgat ggcacaatga acctgatgaa ctgggagtgc gctatccctg gaaagaaggg
      181 gactccatgg gaaggaggct tgttcaagct acggatgctt ttcaaagatg actatccgtc
      241 ctcaccacca aaatgtaaat ttgagccccc actgtttcat ccaaacgtgt atccttctgg
      301 cacagtgtgc ctgtccatcc tggaggaaga caaggactgg aggccagcta tcaccatcaa
      361 acagatctta ttaggaatac aagaacttct aaatgaacca aatattcaag acccagctca
      421 agcagaggcc tacacaattt actgccaaaa cagagtggaa tatgagaaaa gggtccgagc
      481 acaagcgaag aagtttgccc cctcataagg atccgaattc gagctccgtc gacaagcttg
      541 cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa
      601 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
      661 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg
      721 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
      781 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      841 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctt
//
