LOCUS       sequence-86472-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E.coli expression of full length untagged human Uba2 (SAE2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Pichler A, Gast A, Seeler JS, Dejean A, Melchior F
  TITLE     The nucleoporin RanBP2 has SUMO1 E3 ligase activity.
  JOURNAL   Cell. 2002 Jan 11;108(1):109-20.
  PUBMED    11792325
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2002-01-11"; volume: "108(1)"; pages: "109-20"
COMMENT     Sequence Label: pET11d-hUba2
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    62..86
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..123
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag
       61 gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg
      121 agatatacca tggcactgtc gcgggggctg ccccgggagc tggctgaggc ggtggccggg
      181 ggccgggtgc tggtggtggg ggcgggcggc atcggctgcg agctcctcaa gaatctcgtg
      241 ctcaccggtt tctcccacat cgacctgatt gatctggata ctattgatgt aagcaacctc
      301 aacagacagt ttttgtttca aaagaaacat gttggaagat caaaggcaca ggttgccaag
      361 gaaagtgtac tgcagtttta cccgaaagct aatatcgttg cctaccatga cagcatcatg
      421 aaccctgact ataatgtgga atttttccga cagtttatac tggttatgaa tgctttagat
      481 aacagagctg cccgaaacca tgttaataga atgtgcctgg cagctgatgt tcctcttatt
      541 gaaagtggaa cagctgggta tcttggacaa gtaactacta tcaaaaaggg tgtgaccgag
      601 tgttatgagt gtcatcctaa gccgacccag agaacctttc ctggctgtac aattcgtaac
      661 acaccttcag aacctataca ttgcatcgtt tgggcaaagt acttgttcaa ccagttgttt
      721 ggggaagaag atgctgatca agaagtatct cctgacagag ctgaccctga agctgcctgg
      781 gaaccaacgg aagccgaagc cagagctaga gcatctaatg aagatggtga cattaaacgt
      841 atttctacta
//
