LOCUS       sequence-86473-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E.coli expression of untagged mature human SUMO2 (amino acids 1-92).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Flotho A, Werner A, Winter T, Frank AS, Ehret H, Melchior F
  TITLE     Recombinant reconstitution of sumoylation reactions in vitro.
  JOURNAL   Methods Mol Biol. 2012;832:93-110. doi: 10.1007/978-1-61779-474-2_5.
  PUBMED    22350878
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2012"; volume: "832:93-110. doi"; pages: " 
            10.1007/978-1-61779-474-2_5"
COMMENT     Sequence Label: pET11a-SUMO2
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    62..86
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..123
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             131..406
                     /codon_start=1
                     /product="human small ubiquitin-related modifier 3"
                     /label=SUMO3
                     /translation="MSEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKA
                     YCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG"
     terminator      473..520
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(695..700,701..717,718..723))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(750..768)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag
       61 gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg
      121 agatatacat atgtccgagg agaagcccaa ggagggtgtg aagacagaga atgaccacat
      181 caacctgaag gtggccgggc aggacggctc cgtggtgcag ttcaagatca agaggcacac
      241 gccgctgagc aagctgatga aggcctactg cgagaggcag ggcttgtcaa tgaggcagat
      301 cagattcagg ttcgacgggc agccaatcaa tgaaactgac actccagcac agctggagat
      361 ggaggacgag gacaccatcg acgtgttcca gcagcagacg ggaggttagg atccggctgc
      421 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
      481 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
      541 cggatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc
      601 agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga
      661 ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt
      721 caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
      781 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
      841 aacccctatt tgtttatttt tctaaataca ttcaa
//
