LOCUS       sequence-86474-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E.coli expression of untagged mature human SUMO3 (amino acids 1-93).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Flotho A, Werner A, Winter T, Frank AS, Ehret H, Melchior F
  TITLE     Recombinant reconstitution of sumoylation reactions in vitro.
  JOURNAL   Methods Mol Biol. 2012;832:93-110. doi: 10.1007/978-1-61779-474-2_5.
  PUBMED    22350878
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2012"; volume: "832:93-110. doi"; pages: " 
            10.1007/978-1-61779-474-2_5"
COMMENT     Sequence Label: pET11a-SUMO3
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    62..86
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..123
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             131..409
                     /codon_start=1
                     /product="human small ubiquitin-related modifier 2"
                     /label=SUMO2
                     /translation="MADEKPKEGVKTENNDHINLKVAGQDGSVVQFKIKRHTPLSKLMK
                     AYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG"
     primer_bind     complement(462..480)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      476..523
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(698..703,704..720,721..726))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(753..771)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag
       61 gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg
      121 agatatacat atggccgacg aaaagcccaa ggaaggagtc aagactgaga acaacgatca
      181 tattaatttg aaggtggcgg ggcaggatgg ttctgtggtg cagtttaaga ttaagaggca
      241 tacaccactt agtaaactaa tgaaagccta ttgtgaacga cagggattgt caatgaggca
      301 gatcagattc cgatttgacg ggcaaccaat caatgaaaca gacacacctg cacagttgga
      361 aatggaggat gaagatacaa ttgatgtgtt ccaacagcag acgggtggtt aggatccggc
      421 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
      481 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
      541 atccggatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca
      601 tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc
      661 tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc
      721 tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
      781 taatgtcatg ataataatgg tttcttagac gtcaggtggc actt
//
