LOCUS       sequence-86475-e         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human Par1 with N-terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Ishii K, Hein L, Kobilka B, Coughlin SR
  TITLE     Kinetics of thrombin receptor cleavage on intact cells. Relation to 
            signaling.
  JOURNAL   J Biol Chem. 1993 May 5. 268(13):9780-6.
  PUBMED    7683662
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1993 May 5. 268(13):9780-6."
COMMENT     Sequence Label: pBJ FLAG-hPar1
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             52..320
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             342..431
                     /codon_start=1
                     /product="signal sequence from bovine prolactin"
                     /label=prolactin signal sequence
                     /translation="MDSKGSSQKGSRLLLLLVVSNLLLCQGVVS"
ORIGIN
        1 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcctcgatcg aggggctcgc
       61 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
      121 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
      181 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
      241 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
      301 gttctgcgcc gttacagatc caagctcctc gaggatccac catggacagc aaaggttcgt
      361 cgcagaaagg gtcccgcctg ctcctgctgc tggtggtgtc aaatctactc ttgtgccagg
      421 gtgtggtctc cgattacaaa gatgatgatg atgtcgacgc caccttagat ccccggtcat
      481 ttcttctcag gaaccccaat gataaatatg aaccattttg ggaggatgag gagaaaaatg
      541 aaagtgggtt aactgaatac agattagtct ccatcaataa aagcagtcct cttcaaaaac
      601 aacttcctgc attcatctca gaagatgcct ccggatattt gaccagctcc tggctgacac
      661 tctttgtccc atctgtgtac accggagtgt ttgtagtcag cctcccacta aacatcatgg
      721 ccatcgttgt gttcatcctg a
//
