LOCUS       sequence-86478-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human Par2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Bohm SK, Kong W, Bromme D, Smeekens SP, Anderson DC, Connolly A, 
            Kahn M, Nelken NA, Coughlin SR, Payan DG, Bunnett NW
  TITLE     Molecular cloning, expression and potential functions of the human 
            proteinase-activated receptor-2.
  JOURNAL   Biochem J. 1996 Mar 15;314 ( Pt 3)(Pt 3):1009-16. doi: 
            10.1042/bj3141009.
  PUBMED    8615752
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            J."; date: "1996-03-15"; volume: "314 ( Pt 3)(Pt 3):1009-16. doi"; 
            pages: " 10.1042/bj3141009"
COMMENT     Sequence Label: pcDNA3-hPar2
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      86..95
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttgccac catggactac aaggacgacg acgacaagat
      121 gcggagcccc agcgcggcgt ggctgctggg ggccgccatc ctgctagcag cctctctctc
      181 ctgcagtggc accatccaag gaaccaatag atcctctaaa ggaagaagcc ttattggtaa
      241 ggttgatggc acatcccacg tcactggaaa aggagttaca gttgaaacag tcttttctgt
      301 ggatgagttt tctgcatctg tcctcactgg aaaactgacc actgtcttcc ttccaattgt
      361 ctacacaatt gtgtttgtgg tgggtttgcc aagtaacggc atggccctgt gggtctttct
      421 tttccgaact aagaagaagc accctgctgt gatttacatg gccaatctgg ccttggctga
      481 cctcctctct gtcatctggt tccccttgaa gattgcctat cacatacatg gcaacaactg
      541 gatttatggg gaagctcttt gtaatgtgct tattggcttt ttctatggca acatgtactg
      601 ttccattctc ttcatgacct gcctcagtgt gcagaggtat tgggtcatcg tgaaccccat
      661 ggggcactcc aggaagaagg caaacattgc cattggcatc tccctggcaa tatggctgct
      721 gattctgctg gtcaccatcc ctttgtatgt cgtgaagcag accatcttca ttcctgccct
      781 gaacatcacg acctgtcatg atgttttgcc tgagcagctc ttggtgggag acatgttcaa
      841 ttacttcctc tctctggcca ttggggtctt tctgttccca gccttcctca cagcctctgc
      901 ctatgtgctg atgatca
//
