LOCUS       sequence-86487-e         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E.coli expression of HisYFP tagged human Sp100 fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Flotho A, Werner A, Winter T, Frank AS, Ehret H, Melchior F
  TITLE     Recombinant reconstitution of sumoylation reactions in vitro.
  JOURNAL   Methods Mol Biol. 2012;832:93-110. doi: 10.1007/978-1-61779-474-2_5.
  PUBMED    22350878
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2012"; volume: "832:93-110. doi"; pages: " 
            10.1007/978-1-61779-474-2_5"
COMMENT     Sequence Label: pET28a-HisYFP-Sp100
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttgtcga
       61 cgctaatctt ctttacctga ccctcttctt agggatgagc tgcaggtttc ctgaagctct
      121 tctccattac tcaggtctga aaagtcacta ctgctgaaac gtctcttcct gctgggtatt
      181 ctccaagtag atgtacttct agaagtcata ggagccttct caccatgctt gcttctcagt
      241 gccccgcttg aggcttctgc gggcgcctcc tcctcactgg attctgaaaa gtcgtggtct
      301 tgtcctatca ctcttttctt aaaactttct ctgaatgtag ataattttct gaaatccatg
      361 ggctctggta caatctgggg tcgtgagcaa gtggcttctt ggccctcctt ttcatcattt
      421 gattctaaag ggttgtcatt attgatcaca ggctcacttc tcggtgctga gatgaagact
      481 tctaagggct catcaacgtc agtggatcct tcagagtcct cactgctgat gactattatg
      541 tcagatgcct ggttatgatg agttcttgct tgggcttggc actcaacttt tgaattagaa
      601 aatggctttt ccttttttat atccaccagt cgcacagaac aggaattaat ttggattcca
      661 tggttgtgta gctctgcctc tgggctttct tcatcacagg gcaacgggca gggcatctcc
      721 cttccagcat cc
//
