LOCUS       sequence-86488-e         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of mouse Par4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  TITLE     Coughlin Lab Par plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBJKS-mPar4
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             55..323
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     primer_bind     complement(406..422)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(407..423)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             456..545
                     /codon_start=1
                     /product="signal sequence from bovine prolactin"
                     /label=prolactin signal sequence
                     /translation="MDSKGSSQKGSRLLLLLVVSNLLLCQGVVS"
     CDS             complement(795..806)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcctcga tcgaggggct
       61 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
      121 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
      181 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
      241 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
      301 tctgttctgc gccgttacag atccaagctc ctcgctccac cgcggtggcg gccgctctag
      361 aactagtaga tctcccgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct
      421 cgagatccat tgtgctctaa agtcgaggat ccaccatgga cagcaaaggt tcgtcgcaga
      481 aagggtcccg cctgctcctg ctgctggtgg tgtcaaatct actcttgtgc cagggtgtgg
      541 tctccgatta caaagatgat gatgatgtcg cagagggcat ccagaccccc agcatctacg
      601 atgatgtaga gagtaccagg ggaagccatg aaggccctct gggtcccaca gtagaactca
      661 aggagccgaa gtcctcagac aagcctaatc cacgaggcta cccgggcaaa ttctgtgcca
      721 acgacagtga cacgctggag ctcccggcca gctctcaagc actgctgctg gggtgggtcc
      781 ccacgaggct ggtacctgcc ctctatgggc ttgtggtggc tgtggggctg cctgccaatg
      841 ggctggcgct gtgggtgctg gccacaaggg tgccacgcct gccatccacc attctgctca
      901 tgaacctggc agtggctgat ctgctgttgg ccctggtgct gccaccacga ctggcttac
//
