LOCUS       sequence-86495-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E.coli expression of full length human Aos1 (SAE1) with Nterminal 
            His-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Pichler A, Gast A, Seeler JS, Dejean A, Melchior F
  TITLE     The nucleoporin RanBP2 has SUMO1 E3 ligase activity.
  JOURNAL   Cell. 2002 Jan 11;108(1):109-20.
  PUBMED    11792325
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2002-01-11"; volume: "108(1)"; pages: "109-20"
COMMENT     Sequence Label: pET28a-His-hAos1
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..28)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg tcgacggagc
       61 tcgaattcgg atcccgggcc aatgacttca gttttccttg cctggagatg ccttcatgaa
      121 ggaaggggac agggaataca ggtgggcatg ctgcagttgg catctctggg gctgccaaat
      181 cttgagttca cttggggcca aggcactcca caatcccatt ccccttcatg ccatcgaaga
      241 agaagaagtt gttgtgagga gggtcccgct gagacagggc cttcacaatt tcctgtgcca
      301 aaatccctcc aaccaccgca cacactgggg ccatctcgga gaagcagtac ctgacaaagt
      361 cctcaggaag caggtcagga ctaataccca gtgagtcaag cacatcattt cgtatctgga
      421 gcaacaactc agaatcttcc tcatatgtat cagaactggg atctcttcct ttatctgtac
      481 ggaactttaa gagcacttga aggagaaagt agtcggaggt cgtgcgcttc agagcagcct
      541 ttgctttctc actgctccag tccacctcca gggcttcttt aacagggcag aagaccacct
      601 tctttttgac catcgttgtc tcagaagaat caagttttgc tctcttggtg tcgggcccat
      661 cttctactcc ttggctaact ttggcaactt tagttttctc ctctacaaac tcatgctctc
      721 ctagattggc aaatgtgtat ccatggtagc caaaaacatc tcctgtaaag aacttgatgc
      781 tatttttgtg acagatctgg tcaactttaa ctatgacatc cctggagcag caagtcagac
      841 acacagcatc gaattgagtg aaaaatgact ctggttt
//
