LOCUS       sequence-86496-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of full length human Uba2 with Cterminal His-tag in 
            E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Werner A, Moutty MC, Moller U, Melchior F
  TITLE     Performing in vitro sumoylation reactions using recombinant enzymes.
  JOURNAL   Methods Mol Biol. 2009;497:187-99. doi: 
            10.1007/978-1-59745-566-4_12.
  PUBMED    19107418
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2009"; volume: "497:187-99. doi"; pages: " 
            10.1007/978-1-59745-566-4_12"
COMMENT     Sequence Label: pET28b-hUba2-His
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..28)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(77..106)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="ASMTGGQQMG"
ORIGIN
        1 ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg tcgacggagc
       61 tcgaattcgg atcccgaccc atttgctgtc caccagtcat gctagctcca tctaatgcta
      121 tgacatcatc aagctcttcc ttctgttcta tacgtgacct ctttgcactg agattctctt
      181 tctcatctaa tttcctcttg cggcttctct cttcttcact gacgtcggca ttatttgaag
      241 aatcttcttc atccgaatca actatgagaa cgtcatcttg ctcttgagct gtggaggtgg
      301 agggctgagc tccatcatca ctgccattgg ttatgctttt ggcagcatct tcagcttgtt
      361 tgggccccac tttttccggg gcatcaccaa caacttcaaa ttcaacgtcc tttcctaggt
      421 cttcactatg aaggatgttg atcaataaag tatagtcctg gaggaagtca tctgcttgaa
      481 gccggctgcc atttctaatt ccaaattctg acaacttctt gtgattatta gcttccgtct
      541 ctccctcttc ggaagatatt aggattgttc ctttcccatc ttcaatttgg acatctggtg
      601 ctaccatagc aaatttttct ttcactatct tgtcttgtaa ggtgagaaca gtcactttat
      661 ggacattcag ccgcacagtc acctctggct tgctggcaca tacataacaa ttggggttgg
      721 gaggatccag tgcacaaggc acaagaagct tctttcttgg gtttggttgt ttattcaaaa
      781 aaattgttct gcactggtct atttttcctg ataaaatctt caatccttcc aatact
//
