LOCUS       sequence-86498-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses alpha hemolysin under the control of theophylline 
            riboswitch.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Lentini R, Santero SP, Chizzolini F, Cecchi D, Fontana J, 
            Marchioretto M, Del Bianco C, Terrell JL, Spencer AC, Martini L, 
            Forlin M, Assfalg M, Dalla Serra M, Bentley WE, Mansy SS
  TITLE     Integrating artificial with natural cells to translate chemical 
            messages that direct E. coli behaviour.
  JOURNAL   Nat Commun. 2014 May 30;5:4012. doi: 10.1038/ncomms5012.
  PUBMED    24874202
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-05-30"; volume: "5:4012. doi"; pages: " 
            10.1038/ncomms5012"
COMMENT     Sequence Label: JF001A
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..59
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(112..129)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aaaacccctc aagacccgtt tagaggcccc aaggggttat gctagttatt gctcagcggt
       61 ggcagcagcc aactcagctt cctttcgggc tttgttagca gccggatctc agtggtggtg
      121 gtggtggtgc tcgagttagt tggtcatttc ttctttttcc caatcaattt tgtagcgttc
      181 tgaactgcga tccgtccatt tatctttggt attggtgcct ttccaattgg ttgaggtcca
      241 atgcagttga taatcatcac gcacacgttc gtagatcaca tcaatgttgg tctgctgttt
      301 gctggctttg cgatccatgg taataaccgt ggcaaaatcc gggctaaaac cgctggacag
      361 caggcttgag gctttgttcg ggtccagaaa attatccgcc gctttcatac taccgttgcg
      421 ggttttcata aacagctgat tgccataaac cggattccag gaatcgcgat cgtacggacc
      481 ccagttctga ttcaccatgt tgttgaagat aactttccaa cccacttttt tatcggtcgg
      541 actttccaga atggttttga aatccggttg cacgtatttc agcgtatgac caatggacac
      601 attggcgcca atcagaccgc caattttacc cgtatcatca ccggtaacat tgccattaaa
      661 accataggtc agggtactca tatattcttt ggtatcgatg ctattacgcg gataataatc
      721 tgaaatctgc gcc
//
