LOCUS       sequence-86510-e       10626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains soybean miRNA miR3514 recognition sequence 
            (AAGGTCTCTGTCTTAATGGTGA) to produce siRNAs from 3' target sequences 
            and induce RNA silencing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10626)
  AUTHORS   Jacobs TB, Lawler NJ, LaFayette PR, Vodkin LO, Parrott WA
  TITLE     Simple gene silencing using the trans-acting siRNA pathway.
  JOURNAL   Plant Biotechnol J. 2015 Mar 27. doi: 10.1111/pbi.12362.
  PUBMED    25816689
REFERENCE   2  (bases 1 to 10626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2015 Mar 27. doi: 10.1111/pbi.12362."
COMMENT     Sequence Label: p201N 3514
FEATURES             Location/Qualifiers
     source          1..10626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2617..2634)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2617..2633)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2626..2648)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    2829..2853
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4153..4782
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5216..6283
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6349..6543
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6779..6801)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6887..7027
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6942..6961
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7042..7059)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7213..7801)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7293..7312)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7896..8711)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7896..8711)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8619..8638
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8703..8722
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     misc_feature    9004..9028
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    9205..9226
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9241..9246,9247..9264,9265..9271)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9279..9295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9284..9306
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9303..9319
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9303..9319
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(9807..10601)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9918..9937)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     10528..10547
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 cgaagatcga tccttcgcct ggaggagaga aatcagtggc gctgcggctt ttagggtttc
       61 tttgttgatg gaatgagagt gtaagctctg ccagtgccac tttattaggg ttttacaagc
      121 ccttttcttc gtaattgggc ctgacatttt gtgccacttg ggcctttaga gatgaaaatg
      181 tatattgggc ttaagttgac ttgaaggata aattagttta ggatattacg ttttttatga
      241 gaattggtgt gtcggataca tgtatatgat gcattcaaat atatgtattc tagatacatt
      301 taagtttaga tacaatctaa aatgtgtctt taattacagg actgtaacta aaatacttaa
      361 tgtaagaaga atattactcc tttaatagct tttgagtata tctagtctaa catcttttaa
      421 aaaagtctaa tttctttcat ttatttttcg agcaatagca aagtgcataa ttattttttt
      481 cttctagaaa ttcagatttg tttctctaaa ttttgagatt cttttctcaa ttttgtatgt
      541 ctagagaaca atgtgtattt ttcactctag ttggttgtta ctttgttgaa tgttctgata
      601 aaagtatatt gttatttctg aagtagatat aaaccttcat ttggaaatta tacataaatc
      661 aaaatcgtta attatctaga tcaagatata tgcccttttc ctaatgtatt tgatacatgc
      721 acctaatttc actagatgta tcttttctat tttttaaatt atgaatagtt aattttttcc
      781 atatgtgtat ttgatacata cttcatgact ttaaaaaatt aattatatac cagatatatg
      841 tatttaaaat ttgttatgta tttaaagtat gtatatgatt attcgatatt aatctcttcg
      901 atgaaattta aatcgataac tatgtgcttt ggatcgatct gcccactagt gagtcgtatt
      961 acaattcatg actctcttaa ggtagccaaa atcgatcact agtgcggccg cgcgggccca
     1021 atataacaac gacgtcgtaa cagataaagc gaagcttgaa ggtgcatgtg actccgtcaa
     1081 gattacgaaa ccgccaacta ccacgcaaat tgcaattctc aatttcctag aaggactctc
     1141 cgaaaatgca tccaatacca aatattaccc gtgtcatagg caccaagtga caccatacat
     1201 gaacacgcgt cacaatatga ctggagaagg gttccacacc ttatgctata aaacgcccca
     1261 cacccctcct ccttccttcg cagttcaatt ccaatatatt ccattctctc tgtgtatttc
     1321 cctacctctc ccttcaaggt tagtcgattt cttctgtttt tcttcttcgt tctttccatg
     1381 aattgtgtat gttctttgat caatacgatg ttgatttgat tgtgttttgt ttggtttcat
     1441 cgatcttcaa ttttcataat cagattcagc ttttattatc tttacaacaa cgtccttaat
     1501 ttgatgattc tttaatcgta gatttgctct aattagagct ttttcatgtc agatcccttt
     1561 acaacaagcc ttaattgttg attcattaat cgtagattag ggcttttttc attgattact
     1621 tcagatccgt taaacgtaac catagatcag ggctttttca tgaattactt cagatccgtt
     1681 aaacaacagc cttatttttt atacttctgt ggtttttcaa gaaattgttc agatccgttg
     1741 acaaaaagcc ttattcgttg attctatatc gtttttcgag agatattgct cagatctgtt
     1801 agcaactgcc ttgtttgttg attctattgc cgtggattag ggtttttttt cacgagattg
     1861 cttcagatcc gtacttaaga ttacgtaatg gattttgatt ctgatttatc tgtgattgtt
     1921 gactcgacag cggccgcacc ggcgcgaagg tctctgtctt aatggtgagg cgcgccttgg
     1981 atccttccta ggttcgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt
     2041 gtgcttgtaa tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat
     2101 ggatggagaa gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt
     2161 tgttttttct cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt
     2221 tgttttgagt aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt
     2281 aaaacacttg tagttgtacc attatgctta ttcactaggc aacaaatata ttttcagacc
     2341 tagaaaagct gcaaatgtta ctgaatacaa gtatgtcctc ttgtgtttta gacatttatg
     2401 aactttcctt tatgtaattt tccagaatcc ttgtcagatt ctaatcattg ctttataatt
     2461 atagttatac tcatggattt gtagttgagt atgaaaatat tttttaatgc attttatgac
     2521 ttgccaattg attgacaaca tgcatcaatc cgcggttatg actctcttaa ggtagccaaa
     2581 atcgatatct agggataaca gggtaataag cttggcactg gccgtcgttt tacaacgtcg
     2641 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     2701 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     2761 gaatggcgaa tgagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta
     2821 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
     2881 aacggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc
     2941 aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg
     3001 cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct
     3061 aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta
     3121 gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg
     3181 ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc
     3241 aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca
     3301 ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg
     3361 tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg
     3421 agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca
     3481 ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt
     3541 ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt
     3601 ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc
     3661 aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt
     3721 accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct
     3781 tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag
     3841 aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga
     3901 gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac
     3961 cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc
     4021 cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta
     4081 aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg
     4141 caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg
     4201 accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc
     4261 gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta
     4321 accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc
     4381 gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag
     4441 gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc
     4501 gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc
     4561 tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg
     4621 gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc
     4681 actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag
     4741 gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga
     4801 aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg
     4861 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc
     4921 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg
     4981 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt
     5041 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
     5101 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt
     5161 gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgacggtcg
     5221 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
     5281 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
     5341 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
     5401 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
     5461 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
     5521 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
     5581 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc
     5641 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
     5701 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
     5761 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
     5821 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
     5881 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
     5941 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt
     6001 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
     6061 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
     6121 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
     6181 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
     6241 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
     6301 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac
     6361 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
     6421 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
     6481 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
     6541 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct
     6601 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat
     6661 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga
     6721 ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     6781 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     6841 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     6901 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     6961 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     7021 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7081 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7141 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7201 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7261 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7321 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7381 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7441 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7501 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7561 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7621 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     7681 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7741 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7801 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     7861 agggattttg gtcatgcagg atcatgaatt aattcttaga aaaactcatc gagcatcaaa
     7921 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
     7981 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     8041 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     8101 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt
     8161 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     8221 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga
     8281 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
     8341 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
     8401 ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
     8461 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
     8521 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     8581 tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     8641 tataaatcag catccatgtt ggaatttaat cgcggcctag agcaagacgt ttcccgttga
     8701 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
     8761 gatctggatc acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg
     8821 agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa
     8881 catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg
     8941 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
     9001 tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt
     9061 gcggacgttt ttaatgtact gaattaacgc cgaattgctc tagccaatac gcaaaccgcc
     9121 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     9181 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     9241 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     9301 cacaggaaac agctatgaca tgattacgaa ttccttgact agagggtagg cgcggcttaa
     9361 ttaagccgcg ccgtacccca atagtctcga cagacacata gcacctaaca ttattgctta
     9421 aatttactat ttttttacta tattatactc aacccaatga gcataaagac tgtaaaatct
     9481 caaattcctg agaagcatat ttatcgatcc cacagacttg atagttccat aatccatacg
     9541 ctgcagccaa attgctagtg tgttgaacat ttaacacgta gagaactaga aaagatataa
     9601 aactaagatt gatatccaaa atagacgaga acaataagca aaaactctta gttttgaaat
     9661 aaatcaacaa tcccgagggt tgtcacatac atcaaaaacg aaaatccata tagcaaaaaa
     9721 aactctaaat taccgttcga caaaaagaga aaactgatag gacatttgct aaacattaaa
     9781 atcaaaattt ggatcaagcc gaattctcag aagaactcgt caagaaggcg atagaaggcg
     9841 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     9901 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc
     9961 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
    10021 ggcaagcagg catcgccatg ggtcacgacg agatcatcgc cgtcgggcat gcgcgccttg
    10081 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
    10141 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
    10201 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
    10261 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
    10321 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
    10381 cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg
    10441 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
    10501 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
    10561 gcggccggag aacctgcgtg caatccatct tgttcaatca tctcgagacg taccgcgcga
    10621 tgcatt
//
