LOCUS       sequence-86518-e         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector at position 6, small linker insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_6_Linker
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        52..70
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     52..69
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          167..739
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_signal    812..946
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(836..855)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     890..909
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 atgctcgttt agggaaccgc cattctgcct ggggacgtcg gagcaagctt gatttaggtg
       61 acactataga ggtctcatca ggcggatccg gcggcggtac cagtttgaga ccctcatcct
      121 cccgggtaac taagtaagga tctcgaccga tcctgagaac ttcagggtga gtttggggac
      181 ccttgattgt tctttctttt tcgctattgt aaaattcatg ttatatggag ggggcaaagt
      241 tttcagggtg ttgtttagaa tgggaagatg tcccttgtat caccatggac cctcatgata
      301 attttgtttc tttcactttc tactctgttg acaaccattg tctcctctta ttttcttttc
      361 attttctgta actttttcgt taaactttag cttgcatttg taacgaattt ttaaattcac
      421 ttttgtttat ttgtcagatt gtaagtactt tctctaatca cttttttttc aaggcaatca
      481 gggtatatta tattgtactt cagcacagtt ttagagaaca attgttataa ttaaatgata
      541 aggtagaata tttctgcata taaattctgg ctggcgtgga aatattctta ttggtagaaa
      601 caactacacc ctggtcatca tcctgccttt ctctttatgg ttacaatgat atacactgtt
      661 tgagatgagg ataaaatact ctgagtccaa accgggcccc tctgctaacc atgttcatgc
      721 cttcttctct ttcctacagc tcctgggcaa cgtgctggtt gttgtgctgt ctcatcattt
      781 tggcaaagaa ttaattcctc gacggatcgt agatccagac atgataagat acattgatga
      841 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
      901 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
      961 cattcatttt atgtttcagg
//
