LOCUS       sequence-86519-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Component of the Co-Driver binary site-specific recombinase system, 
            generates functional rox60-71Cre upon removal of an internal STOP 
            cassette by Dre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, 
            Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P
  TITLE     Binary recombinase systems for high-resolution conditional 
            mutagenesis.
  JOURNAL   Nucleic Acids Res. 2014 Jan 9.
  PUBMED    24413561
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jan 9."
COMMENT     Sequence Label: pCAG-Roxed-Cre
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             83..103
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    228..259
                     /label=rox
                     /bound_moiety="Dre recombinase"
     polyA_signal    447..581
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    701..835
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 accatgttca tgccttcttc tttttcctac agatccttaa ttaataatac gactcactat
       61 agggggtcga cccgccacca tgcccaagaa gaagaggaag gtgacctctg atgaagtcag
      121 gaagaacctg atggacatgt tcagggacag gcaggccttc tctgaacaca cctggaagat
      181 gctcctgtct gtgtgcagat cctgggctgc ctggtgcaag ctgaacgtaa ctttaaataa
      241 ttggcattat ttaaagttaa tgtatgcgct acgaagttat tcgcgatgaa taaatgaaag
      301 cttgcagatc tgcgactcta gaggatctgc gactctagag gatcataatc agccatacca
      361 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
      421 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
      481 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
      541 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgcgactct agaggatcat
      601 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
      661 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
      721 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
      781 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctgcga
      841 ctctagagga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
      901 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
      961 tttattgca
//
