LOCUS       sequence-86524-e        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Srinivas S, Watanabe T, Lin CS, William CM, Tanabe Y, Jessell TM, 
            Costantini F
  TITLE     Cre reporter strains produced by targeted insertion of EYFP and ECFP
            into the ROSA26 locus.
  JOURNAL   BMC Dev Biol. 2001;1:4. doi: 10.1186/1471-213x-1-4. Epub 2001 Mar 
            27.
  PUBMED    11299042
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2001"; volume: "1:4. doi"; pages: " 
            10.1186/1471-213x-1-4. Epub 2001 Mar 27"
COMMENT     Sequence Label: pROSA26-PA
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(103..675)
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     primer_bind     complement(750..769)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 tatacactta aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc
       61 aattatgtca caccacagaa gtaaggttcc ttcacaaaga gatcgcctga cacgatttcc
      121 tgcacaggct tgagccatat actcatacat cgcatcttgg ccacgttttc cacgggtttc
      181 aaaattaatc tcaagttcta cgcttaacgc tttcgcctgt tcccagttat taatatattc
      241 aacgctagaa ctcccctcag cgaagggaag gctgagcact acacgcgaag caccatcacc
      301 gaaccttttg ataaactctt ccgttccgac ttgctccatc aacggttcag tgagacttaa
      361 acctaactct ttcttaatag tttcggcatt atccactttt agtgcgagaa ccttcgtcag
      421 tcctggatac gtcactttga ccacgcctcc agcttttcca gagagcgggt tttcattatc
      481 tacagagtat cccgcagcgt cgtatttatt gtcggtacta taaaaccctt tccaatcatc
      541 gtcataattt ccttgtgtac cagattttgg cttttgtata cctttttgaa tggaatctac
      601 ataaccaggt ttagtcccgt ggtacgaaga aaagttttcc atcacaaaag atttagaaga
      661 atcaacaaca tcatcaggat ccatggcgag gacctgcagg tcgaaaggcc cggagatgag
      721 gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg tgcagaatgc cgggcctccg
      781 gaggaccttc gggcgcccgc cccgcccctg agcccgcccc tgagcccgcc cccggaccca
      841 ccccttccca gcctctgagc ccagaaagcg aaggagcaaa gctgctattg gccgctgccc
      901 caaaggccta cccgcttcca ttgctcagcg gtgctgtcca tctgcacgag actagtgaga
      961 cgtgctactt ccatttgtca cgtcctgcac gacgcgagct gcggggcggg ggggaacttc
     1021 ctgactaggg gaggagtaga aggtggcgcg aaggggcca
//
