LOCUS       sequence-86526-e         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLEX switch empty backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Atasoy D, Aponte Y, Su HH, Sternson SM
  TITLE     A FLEX switch targets Channelrhodopsin-2 to multiple cell types for 
            imaging and long-range circuit mapping.
  JOURNAL   J Neurosci. 2008 Jul 9. 28(28):7025-30.
  PUBMED    18614669
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2008 Jul 9. 28(28):7025-30."
COMMENT     Sequence Label: pJ241-FLEX
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    76..109
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(116..149)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(212..245)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(262..295)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(502..521)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(527..546)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        588..679
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gttatgggtc gatggtgaga tctggactag agggtcgatg gtgatgcttg gatcaaaata
       61 ggaagaatcc caatgataac ttcgtataaa gtatcctata cgaagttata ctagtataac
      121 ttcgtatagc atacattata cgaagttatg ggtcgatggt gatgcttggc aattcgggtc
      181 gatggtgaag cattggtctt cctattttga tataacttcg tataggatac tttatacgaa
      241 gttatgggga tccccacttc agaagttcct atactttcta gagaatagga acttcactat
      301 agagtcgaat aagggcgaca ccccctaatt agcccgggcg aaaggcccag tctttcgact
      361 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcatgggg agtccccaca
      421 ctaccatcgg cgctacggcg tttcacttct gagttcggca tggggtcagg tgggaccacc
      481 gcgctactgc cgccaggcaa acaaggggtg ttatgagcca tattcaggta taaatgggct
      541 cgcgataatg ttcagaattg gttaattggt tgtaacactg acccctattt gtttattttt
      601 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      661 atattgaaaa aggaagaata tgagccatat tc
//
