LOCUS       sequence-86531-e        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Srinivas S, Watanabe T, Lin CS, William CM, Tanabe Y, Jessell TM, 
            Costantini F
  TITLE     Cre reporter strains produced by targeted insertion of EYFP and ECFP
            into the ROSA26 locus.
  JOURNAL   BMC Dev Biol. 2001;1:4. doi: 10.1186/1471-213x-1-4. Epub 2001 Mar 
            27.
  PUBMED    11299042
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2001"; volume: "1:4. doi"; pages: " 
            10.1186/1471-213x-1-4. Epub 2001 Mar 27"
COMMENT     Sequence Label: pROSA26-PA
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        288..787
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(323..346)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     720..739
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 atactaactc cactgggtgg atttttaatt cagtcagtag tcttaagtgg tctttattgg
       61 cccttcatta aaatctactg ttcactctaa cagaggctgt tggtactagt ggcacttaag
      121 caacttccta cggatatact agcagattaa gggtcaggga tagaaactag tctagcgttt
      181 tgtataccta ccagctttat actaccttgt tctgatagaa atatttcagg acatctagct
      241 tatcgatacc gtcgacggta tcgataagct tgatatcgaa ttctaccggg taggggaggc
      301 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
      361 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
      421 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
      481 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
      541 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
      601 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
      661 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
      721 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
      781 cctttcgacc tgcaggtcct cgccatggat cctgatgatg ttgttgattc ttctaaatct
      841 tttgtgatgg aaaacttttc ttcgtaccac gggactaaac ctggttatgt agattccatt
      901 caaaaaggta tacaaaagcc aaaatctggt acacaaggaa attatgacga tgattggaaa
      961 gggttttata gtaccgacaa taaatacgac gctgcgggat actctgta
//
