LOCUS       sequence-86533-e         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Component of the Co-Driver binary site-specific recombinase system, 
            generates functional rox60-71Cre upon removal of an internal STOP 
            cassette by Dre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, 
            Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P
  TITLE     Binary recombinase systems for high-resolution conditional 
            mutagenesis.
  JOURNAL   Nucleic Acids Res. 2014 Jan 9.
  PUBMED    24413561
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jan 9."
COMMENT     Sequence Label: pCAG-Roxed-Cre
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    96..207
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     196..213
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(236..259)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc cccaccccac
       61 cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca ttttattagg
      121 aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa
      181 cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgagctctg acctacttat
      241 cgtcgtcatc cttgtaatcg tccccatcct cgagcagcct caccatggcc ccagtctcag
      301 agtccaggtt tctgatgtag ttcatcacaa tgttcacatt ggtccagcca ccagcctgca
      361 tgatttcagg gatggacaca ccagccctgg ccatgtccct ggcagcaccc actctggcag
      421 agtggccaga ccaggccagg tatctctgcc cagagtcatc cttggcacca tagatcaggc
      481 ggtgggtggc ctcaaagatc ccttccaggg cccgggtgga cagttgggag gtggcagaag
      541 gggcagccac accattcttt ctgacccggc agaacaggta gttgttgggg tcatcagcca
      601 caccagacac agagatccat ctctccacca gcttggtaac ccccagggac agggccttct
      661 ccacaccagc tgtggacacc agggtcttgg tcctgccaa
//
