LOCUS       sequence-86534-e        1151 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Strauss KM, Martins LM, Plun-Favreau H, Marx FP, Kautzmann S, Berg 
            D, Gasser T, Wszolek Z, Muller T, Bornemann A, Wolburg H, Downward 
            J, Riess O, Schulz JB, Kruger R
  TITLE     Loss of function mutations in the gene encoding Omi/HtrA2 in 
            Parkinson's disease.
  JOURNAL   Hum Mol Genet. 2005 Aug 1. 14(15):2099-111.
  PUBMED    15961413
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2005 Aug 1. 14(15):2099-111."
COMMENT     Sequence Label: pET20b-delta133HtrA2 G399S
FEATURES             Location/Qualifiers
     source          1..1151
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(40..57)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             1049..1071
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1102..1121)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1103..1121)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctcagcttcc tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc
       61 gagttctgtg acctcagggg tcacatataa ggtcagtgtt tctcgtcccc gccggatctg
      121 cactgccaac tgggattggg ttcgaacagc ttcataaaca tcttcagcat tttgtaccat
      181 ctgctcccca atggccaaaa tcacatcacc aggccgcaga ccagcccggt gtgcagggga
      241 gctcaggatg actttatgga tgagtacacc atgctgaaca tcgggaaagc ttggttctcg
      301 aagctgtagt tcagcaagga tgctgggact cagggtcagc atcatcaccc caatgtagcg
      361 ccgctgggac ccactgattc cggaggagga attcttcttt tccccacgat gcagaaactc
      421 tcgaagacga tcagaaggga tggcaaagga gattccagct gtgaccttca tggtgttcac
      481 tccaatcacc tccccatcca ggttaaccag gggacctcca gagtttccaa aatcaatagc
      541 tgcatcagtt tgaatgtatt ccacattggt ttgcggaagt cccaggtctc tggctggacg
      601 ctgagcagag ctaacaatgc cggatgtgat cgtgttctgc agtgcaaagg gacttcccat
      661 tgcaacaaca aactcccctt gccggacatc agctgagcgt cccagaggca gcgtggggag
      721 aggctcctta gtctgaatcc tcagcgttgc gatgtctgcc acgggatcca cagctgtgac
      781 cacggcctca tacgtgtcgc cgcttagcag tctcacacgg actctgcgcc gatcagccac
      841 cacatgggcg ttggtgacaa tgagcccatc ggcagccacc acgaaccctg agccgttcga
      901 gatagggacc tcgcggccca agaaagggtg ccggtccagg atctcgatat agaccacggc
      961 aggtgctgtc ttctccacca catctgcgat gaagttgtac tgactccggg gagaagcggg
     1021 cggcgggcta gggacggcca tatgtatatc tccttcttaa agttaaacaa aattatttct
     1081 agagggaaac cgttgtggtc tccctatagt gagtcgtatt aatttcgcgg gatcgagatc
     1141 tcgggcagcg t
//
