LOCUS       sequence-86538-e        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of mouse PRDM16 fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Kajimura S, Seale P, Tomaru T, Erdjument-Bromage H, Cooper MP, Ruas 
            JL, Chin S, Tempst P, Lazar MA, Spiegelman BM
  TITLE     Regulation of the brown and white fat gene programs through a 
            PRDM16/CtBP transcriptional complex.
  JOURNAL   Genes Dev. 2008 May 15;22(10):1397-409. doi: 10.1101/gad.1666108.
  PUBMED    18483224
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2008-05-15- 15"; volume: "22"; 
            issue: "10"; pages: "1397-409"
COMMENT     Sequence Label: GST-PRDM16 880-1038
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(585..607)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(752..770)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        838..943
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatctcaatg gatctggttc gcgtggatcc ccaggaattc ccgtgggagt cctgaaagag
       61 aagtacctgc ggccgtcccc acttctgttc cacccccaga tgtcagccat agaaaccatg
      121 acggagaagc tggagagctt tgcagccatg aaggccgact caggcagctc cctgcagccc
      181 ctgcctcacc acccgttcaa cttccgctcc ccacccccaa cgctctcgga tcccatcctc
      241 aggaagggga aggagagata cacgtgcagg tactgtggca agatcttccc cagatctgca
      301 aatctcacaa gacatctgag gacacacaca ggggagcagc catacaggtg caagtactgt
      361 gaccggtcat tcagcatctc ctccaacctc cagcggcacg tgaggaacat ccacaacaaa
      421 gagaagccgt tcaagtgcca tctgtgcaac cgctgcttcg ggcagcagac caacctagac
      481 cggcacctga agaagcacga acacgagggc gcaccatgac tcgagcggcc gcatcgtgac
      541 tgactgacga tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
      601 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
      661 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
      721 agcggagtgt ataattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
      781 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
      841 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
      901 taaccctgat aaatgcttca ataatattga aaaaaggaag agtatgagta ttcaacattt
      961 ccgtgtcgcc ctttattccc ttttttgcgg catttttgcc
//
