LOCUS       sequence-86541-e         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of mouse PRDM16 fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Kajimura S, Seale P, Tomaru T, Erdjument-Bromage H, Cooper MP, Ruas 
            JL, Chin S, Tempst P, Lazar MA, Spiegelman BM
  TITLE     Regulation of the brown and white fat gene programs through a 
            PRDM16/CtBP transcriptional complex.
  JOURNAL   Genes Dev. 2008 May 15;22(10):1397-409. doi: 10.1101/gad.1666108.
  PUBMED    18483224
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2008-05-15- 15"; volume: "22"; 
            issue: "10"; pages: "1397-409"
COMMENT     Sequence Label: GST-PRDM16 1039-1176
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(515..537)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(682..700)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        768..872
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcaatggatc tggttcgcgt ggatccgtga gccagcactc cggggtgctc acgaaccacc
       61 tgggcaccag cgcctcctcc cccacctccg agtcggacaa ccatgcactt ttagatgaga
      121 aggaagattc ttacttctcc gagatccgaa acttcatcgc caacagcgag atgaaccagg
      181 catccactcg aatggacaaa cggcctgaga tccaagacct ggacagtaac ccaccgtgtc
      241 caggctcagc cagtgcaaag ccagaggacg tagaggagga ggaagaggag gagctggagg
      301 aagaggatga tgacagctta gccgggaagt cacaggagga cacggtgtcc cccacacctg
      361 agccccaagg agtctatgaa gatgaagagg atgaggaacc acccagcctg accatgggct
      421 ttgaccatac ccggaggcat atgcaatgac tcgagcggcc gcatcgtgac tgactgacga
      481 tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
      541 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
      601 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
      661 ataattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
      721 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
      781 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      841 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
      901 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcaccccaga aacgctggtg
      961 aaattaaaga tgctga
//
