LOCUS       sequence-86543-e        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows expression of the fission yeast NatB complex - 
            chloramphenicol marker .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Johnson M, Coulton AT, Geeves MA, Mulvihill DP
  TITLE     Targeted amino-terminal acetylation of recombinant proteins in E. 
            coli.
  JOURNAL   PLoS One. 2010 Dec 23;5(12):e15801. doi: 
            10.1371/journal.pone.0015801.
  PUBMED    21203426
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-12-23"; volume: "5(12):e15801. doi"; pages: " 
            10.1371/journal.pone.0015801"
COMMENT     Sequence Label: pNatB (pACYCduet-naa20-naa25)
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     111..130
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        111..129
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    130..154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             210..227
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg
       61 gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaaat taatacgact
      121 cactataggg gaattgtgag cggataacaa ttcccctgta gaaataattt tgtttaactt
      181 taataaggag atataccatg ggcagcagcc atcaccatca tcaccacagc caggatccga
      241 attcgagctc ggcgcgcctg caggtcgaca tgcgtcgttc tgggagtaaa gaatcaacaa
      301 ttgtttattc agcgttatcc ttggctcagg caggacgtgg tcccgaggct ctagcacttt
      361 tagaaccgtt aaaatcgact cccatcaaca gcttggaact tttagacatt atacaagctg
      421 tttatgatga tcaaaagaag ggagaagaat cgtttgtatt ttgggaaaag ttcttgcaaa
      481 cttatggaaa gcaggaaaaa aacttattag cttacttcaa agcatctatt cgcatcaaat
      541 cattgtctca ccaaagaaag gctgctgtag aattgcaaaa gaactttcct tctaggaaac
      601 ataccttatg ggttatctca tctttgtatc ttttgtccaa aaagtcggaa aatgaggttg
      661 aacaacgttt gctgaaagca ctagctgaaa aaactgctaa actcattttc gaaaaaccaa
      721 caggttatat cgattcttgt gaggagtttc atctttattt agacgtgttg cttttagtgg
      781 gagacaagga cagagcgtta gatgctttaa tacatcaaga tgctgatcgc tttgttgatg
      841 ctgacgccga ccttcttttg aggaagcttg aattgcttgc ttcctgtgcg cgttgggatt
      901 ctttattcac gttttcgctt tctcttttcc aaactgggaa tactgattgg aaggtttgca
      961 aagctttgct ggatagtgct tccaatgatg attcaaaatt agtcccatta aaggattgta
     1021 tattgaaagc gttgtcaacc agcagcacaa aaagaaa
//
