LOCUS       sequence-86553-e         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral cDNA/shRNA gateway destination vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Radulovich N, Leung L, Tsao MS
  TITLE     Modified gateway system for double shRNA expression and Cre/lox 
            based gene expression.
  JOURNAL   BMC Biotechnol. 2011 Mar 22;11:24.
  PUBMED    21418658
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2011-03-22"; volume: "11"; pages: "24"
COMMENT     Sequence Label: pLKO.DEST.EGFP
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    168..289
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(179..198)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     233..252
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             361..594
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(729..750)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca
       61 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
      121 ggcgggatgg gcggagttag gggcgggata gctagagcca gacatgataa gatacattga
      181 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
      241 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagttc ctctcactct
      301 ctgatattca tttctttgca agttataaat actgaataat aagatgacat gaactactac
      361 tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac
      421 acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca
      481 gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc
      541 cagtacaagc aaaaagcaga tcttgtcttc gttgggagtg aattagccct tccagtcccc
      601 ccttttcttt taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag
      661 gtaccgatgc atatgcggcc gctttacttg tacagctcgt ccatgccgag agtgatcccg
      721 gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc
      781 agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
      841 ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg
      901 atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
      961 ctgttgtagt tgtactccag cttgtgccc
//
