LOCUS       sequence-86558-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains an ADHpr driven chimeric protein with SNC1, 
            N-terminal GFP and C-terminal SUC2. It contains flanking homology to
            SUC2, allowing optional integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Burston HE, Maldonado-Baez L, Davey M, Montpetit B, Schluter C, 
            Wendland B, Conibear E
  TITLE     Regulators of yeast endocytosis identified by systematic 
            quantitative analysis.
  JOURNAL   J Cell Biol. 2009 Jun 15. 185(6):1097-110.
  PUBMED    19506040
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2009 Jun 15. 185(6):1097-110."
COMMENT     Sequence Label: pHB4
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(20..38)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    79..582
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        619..723
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
       61 ataatggttt cttagacgga tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat
      121 gatggaataa tttgggaatt tactctgtgt ttatttattt ttatgttttg tatttggatt
      181 ttagaaagta aataaagaag gtagaagagt tacggaatga agaaaaaaaa ataaacaaag
      241 gtttaaaaaa tttcaacaaa aagcgtactt tacatatata tttattagac aagaaaagca
      301 gattaaatag atatacattc gattaacgat aagtaaaatg taaaatcaca ggattttcgt
      361 gtgtggtctt ctacacagac aagatgaaac aattcggcat taatacctga gagcaggaag
      421 agcaagataa aaggtagtat ttgttggcga tccccctaga gtcttttaca tcttcggaaa
      481 acaaaaacta ttttttcttt aatttctttt tttactttct atttttaatt tatatattta
      541 tattaaaaaa tttaaattat aattattttt atagcacgtg atgaaaagga cccaggtggc
      601 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      661 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      721 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      781 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      841 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
      901 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
//
