LOCUS       sequence-86559-e        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains an ADHpr driven chimeric protein with SNC1, 
            N-terminal GFP and C-terminal SUC2. It contains flanking homology to
            SUC2, allowing optional integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Burston HE, Maldonado-Baez L, Davey M, Montpetit B, Schluter C, 
            Wendland B, Conibear E
  TITLE     Regulators of yeast endocytosis identified by systematic 
            quantitative analysis.
  JOURNAL   J Cell Biol. 2009 Jun 15. 185(6):1097-110.
  PUBMED    19506040
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2009 Jun 15. 185(6):1097-110."
COMMENT     Sequence Label: pHB4
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(49..621)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     promoter        complement(622..965)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 acttatttaa taataaaaat cataaatcat aagaaattcg cctcgagatt aggggcaggg
       61 catgctcatg tagagcgcct gctcgccgtc cgaggcggtg ccgtcgtaca gggcggtgtc
      121 caggccgcag agggtgaacc ccatccgccg gtacgcgtgg atcgccggtg cgttgacgtt
      181 ggtgacctcc agccagaggt gcccggcgcc ccgctcgcgg gcgaactccg tcgcgagccc
      241 catcaacgcg cgcccgaccc cgtgcccccg gtgctccggg gcgacctcga tgtcctcgac
      301 ggtcagccgg cggttccagc cggagtacga gacgaccacg aagcccgcca ggtcgccgtc
      361 gtccccgtac gcgacgaacg tccgggagtc cgggtcgccg tcctccccgt cgtccgattc
      421 gtcgtccgat tcgtcgtcgg ggaacacctt ggtcaggggc gggtccaccg gcacctcccg
      481 cagggtgaag ccgtccccgg tggcggtgac gcggaagacg gtgtcggtgg tgaaggaccc
      541 atccagtgcc tcgatggcct cggcgtcccc cgggacactg gtgcggtacc ggtaagccgt
      601 gtcgtcaaga gtggtaccca tggttgttta tgttcggatg tgatgtgaga actgtatcct
      661 agcaagattt taaaaggaag tatatgaaag aagaacctca gtggcaaatc ctaacctttt
      721 atatttctct acaggggcgc ggcgtgggga caattcaacg cgtctgtgag gggagcgttt
      781 ccctgctcgc aggtctgcag cgaggagccg taatttttgc ttcgcgccgt gcggccatca
      841 aaatgtatgg atgcaaatga ttatacatgg ggatgtatgg gctaaatgta cgggcgacag
      901 tcacatcatg cccctgagct gcgcacgtca agactgtcaa ggagggtatt ctgggcctcc
      961 atgtcgctgg ccgggtgacc cggcggggac gaggcaagcg cacaagaaca agagaatgtt
     1021 ttgaagaata atacatatct atttatatgt ttcttttcag gaggaa
//
