LOCUS       sequence-86560-e        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains an ADHpr driven chimeric protein with SNC1, 
            N-terminal GFP and C-terminal SUC2. It contains flanking homology to
            SUC2, allowing optional integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Burston HE, Maldonado-Baez L, Davey M, Montpetit B, Schluter C, 
            Wendland B, Conibear E
  TITLE     Regulators of yeast endocytosis identified by systematic 
            quantitative analysis.
  JOURNAL   J Cell Biol. 2009 Jun 15. 185(6):1097-110.
  PUBMED    19506040
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2009 Jun 15. 185(6):1097-110."
COMMENT     Sequence Label: pHB4
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..27
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8..26
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcgcgcgtaa tacgactcac tatagggcga attgggtacc ggtatccatt tccctcacta
       61 cttctttttg agaacacttt tacgtagaag acaacgcttt ttttttttta caatatggta
      121 tacttctact taatgctttt aaattttcac ataactaata ctttacccgg tttcattgtg
      181 tcagtaacaa aacagataaa ggcaaaaagg tccatcctag tagtgtaagg caactacatt
      241 acgattgtgg gtttattacg tgaaaaaatg atgtctattg caaagtagta aaaattttta
      301 agagaattat acttatataa agtacataaa gaacaaattc caggtaactg gggtcgggag
      361 aaaataaaaa taaaaataca ttttcaatgg tttggaggta ttctttgaaa tcataaagtt
      421 ttacattcgt cactcgttag ctaaagccct ttagaatggc ttttgaaaaa aataaaaaag
      481 acaataagtt ttataacctc tattttactt cccttacttg gaacttgtca atgtagaaca
      541 aattatcgac accagtggtc atgttcacag atcctagagc gttaccggtg gtcatgaagt
      601 aggtatttgt agaaaccaca tctccatcgt tgaagtacaa ttccaagatg ttttgatcca
      661 gtaggccgta cactttatag taacttaggt cgttctcaga cttgaatggt tggttgttga
      721 cagacattct gtttgtgaaa tatgggttct ccttgacaaa cttgacctta gagttaccac
      781 ggtccaaaaa gaaggaagaa gcactgactt caaaacccat tctcaaatat tcttcaggat
      841 cttctaaacc cttgaaccaa agtgataagt cggcaaagac ggatttggat atggtttgtg
      901 tggtgttaac agcgtaaacc aactcaaact ctagggtacc agtcgagttg ctcaaatcga
      961 cattgtaaga attggcctta gttagagttg tgttagtagc aaaacgagac cagggaccag
     1021 cattactaat gttcaatatt ggttcggctt tcaaattgat caattcagtc tctggattag
     1081 cttgatattc
//
