LOCUS       sequence-86564-e        6472 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  transgene expression driven by inducible ALPHA MHC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Sanbe A, Gulick J, Hanks MC, Liang Q, Osinska H, Robbins J
  TITLE     Reengineering inducible cardiac-specific transgenesis with an 
            attenuated myosin heavy chain promoter.
  JOURNAL   Circ Res. 2003 Apr 4;92(6):609-16. Epub 2003 Mar 6.
  PUBMED    12623879
REFERENCE   2  (bases 1 to 6472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Circ Res.";
            date: "2003-04-4"; volume: "92(6)"; pages: "609-16. Epub 2003 Mar 6"
COMMENT     Sequence Label: pJG/ Inducible ALPHA MHC
FEATURES             Location/Qualifiers
     source          1..6472
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..25
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(4315..4333,4334..4356,4357..4375,4376..4398,4399..4417,
                     4418..4440,4441..4459,4460..4482,4483..4501,4502..4524,
                     4525..4543,4544..4566,4567..4585)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     polyA_signal    5776..6398
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5975..5994)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(6413..6429)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcggccgctc tagaactagt ggatcctgca aggtcacaca agggtctcca cccaccaggt
       61 gccctagtct caatttcagt ttccatgcct tgttctcaca atgctggcct ccccagagct
      121 aatttggact ttgtttttat ttcaaaaggg cctgaatgag gagtagatct tgtgctaccc
      181 agctctaagg gtgcccgtga agccctcaga cctggagcct ttgcaacagc cctttaggtg
      241 gaagcagaat aaagcaattt tccttaaagc caaaatcctg cctctagact cttcttctct
      301 gacctcggtc cctgggctct agggtgggga ggtggggctt ggaagaagaa ggtggggaag
      361 tggcaaaagc cgatccctag ggccctgtga agttcggagc cttccctgta cagcactggc
      421 tcatagatcc tcctccagcc aaacatagca agaagtgata cctcctttgt gacttcccca
      481 ggcccagtac ctgtcaggtt gaaacaggat ttagagaagc ctctgaactc acctgaactc
      541 tgaagctcat ccaccaagca agcacctagg tgccactgct agttagtatc ctacgctgat
      601 aatatgcaga gctgggccac agaagtcctg gggtgtagga actgaccagt gacttttcag
      661 tcggcaaagg tatgaccccc tcagcagatg tagtaatgtc cccttagatc ccatcccagg
      721 caggtctcta agaggacatg ggatgagaga tgtagtcatg tggcattcca aacacagcta
      781 tccacagtgt cccttgcccc ttccacttag ccaggaggac agtaacctta gcctatcttt
      841 cttcctcccc atcctcccag gacacacccc ctggtctgca gtattcattt cttccttcac
      901 gtcccctctg tgacttccat ttgcaaggct tttgacctct gcagctgctg gaagatagag
      961 tttggcccta ggtgtggcaa gccatctcaa gagaaagcag acaacagggg gaccagattt
     1021 tggaaggatc aggaactaaa tcactggcgg gcctgggggt agaaaaaaga gtgagtgagt
     1081 ccgctccagc taagccaagc tagtccccga gatactctgc cacagctggg ctgctcgggg
     1141 tagctttagg aatgtgggtc tgaaagacaa tgggattgga agacatctct ttgagtctcc
     1201 cctcaacccc acctacagac acactcgtgt gtggccagac tcctgttcaa cagccctctg
     1261 tgttctgacc actgagctag gcaaccagag catgggccct gtgctgagga tgaagagttg
     1321 gttaccaata gcaaaaacag caggggaggg agaacagaga acgaaataag gaaggaagaa
     1381 ggaaaggcca gtcaatcaga tgcagtcaga agagatggga agccaacaca cagcttgagc
     1441 agaggaaaca gaaaagggag agattctggg cataaggagg ccacagaaag aagagcccag
     1501 gccccccaag tctcctcttt ataccctcat cccgtctccc aattaagccc actcttcttc
     1561 ctagatcaga cctgagctgc agcgaagaga cccgtaggga ggatcacact ggatgaagga
     1621 gatgtgtgga gaagtccagg gcaacctaag agccagagcc taaaagagca agagataaag
     1681 gtgcttcaaa ggtggccagg ctgtgcacac agagggtcga ggactggtgg tagagcctca
     1741 agataaggat gatgctcaga atgggcgggg ggggggattc tggggggggg agagagaagg
     1801 tgagaaggag cctggaacag agaatctgga agcgctggaa acgataccat aaagggaaga
     1861 acccaggcta cctttagatg taaatcatga aagacaggga gaagggaagc tggagagagt
     1921 agaaggaccc cggggcaaga catggaagca aggacaagcc aggttgagcg ctccgtgaaa
     1981 tcagcctgct gaaggcagag ccctggtatg agcaccagaa cagcagaggc tagggttaat
     2041 gtcgagacag ggaacagaag gtagacacag gaacagacag agacggggga gccaggtaac
     2101 aaaggaatgg tccttctcac ctgtggccag agcgtccatc tgtgtccaca tactctagaa
     2161 tgttcatcag actgcagggc tggcttggga ggcagctgga aagagtatgt gagagccagg
     2221 ggagacaagg gggcctagga aaggaagaag agggcaaacc aggccacaca agagggcaga
     2281 gcccagaact gagttaactc cttccttgtt gcatcttcca taggaggcag tgggaactct
     2341 gtgaccacca tcccccatga gcccccacta cccataccaa gtttggcctg agtggcattc
     2401 taggttccct gaggacagag cctggccttt gtctcttgga cctgacccaa gctgacccaa
     2461 tgttctcagt acctttgaat gccctcaaga gcttgagaac caggcagtga catattaggc
     2521 catgggctaa ccctggagct tgcacacagg agcctcaagt gacctccagg gacacagctg
     2581 cagacaggtg gcctttatcc ccaaagagca accatttggc ataggtggct gcaaatggga
     2641 atgcaaggtt gaatcaggtc ccttcaagaa tactgcatgc aagacctaag acccctggag
     2701 agaggggtat gctcctgccc ccacccacca taaggggagt gaactatcct agggggctgg
     2761 cgaccttggg gagacaccac attactgaga gtgctgagcc cagaaaaact gaccgccctg
     2821 tgtcctgccc acctccacac tctagagcta tattgagagg tgacagtaga tagggtggga
     2881 gctggtagca gggagagtgt tcctgggtgt gagggtgtag gggaaagcca gagcagggga
     2941 gtctggcttt gtctcctgaa cacaatgtct acttagttat aacaggcatg acctgctaaa
     3001 gacccaacat ctacgacctc tgaaaagaca gcagccctgg aggacagggg ttgtctctga
     3061 gccttgggtg cttgatggtg ccacaaagga gggcatgagt gtgagtataa ggccccagga
     3121 gcgttagaga agggcacttg ggaaggggtc agtctgcaga gcccctatcc atggaatctg
     3181 gagcctgggg ccaactggtg taaatctctg ggcctgccag gcattcaaag cagcacctgc
     3241 atcctctggc agcctgggga ggcggaaggg agcaaccccc cacttatacc ctttctccct
     3301 cagccccagg attaacacct ctggccttcc cccttcccac ctcccatcag gagtggaggg
     3361 ttgcagaggg agggtaaaaa cctacatgtc caaacatcat ggtgcacgat atatggatca
     3421 gtatgtgtag aggcaagaaa ggaaatctgc aggcttaact gggttaatgt gtaaagtctg
     3481 tgtgcatgtg tgtgtgtctg actgaaaacg ggcatggctg tgcagctgtt cagttctgtg
     3541 cgtgaggtta ccagactgca ggtttgtgtg taaattgccc aaggcaaagt gggtgaatcc
     3601 cttccatggt ttaaagagat tggatgatgg cctgcatctc aaggaccatg gaaaatagaa
     3661 tggacactct atatgtgtct ctaagctaag gtagcaaggt ctttggagga cacctgtcta
     3721 gagatgtggg caacagagac tacagacagt atctgtacag agtaaggaga gagaggaggg
     3781 ggtgtagaat tctcttacta tcaaagggaa actgagtcgt gcacctgcaa agtggatgct
     3841 ctccctagac atcatgactt tgtctctggg gagccagcac tgtggaactt caggtctgag
     3901 agagtaggag gctcccctca gcctgaagct atgcagatag ccagggttga aagggggaag
     3961 ggagagcctg ggatgggagc ttgtgtgttg gaggcagggg acagatatta agcctggaag
     4021 agaaggtgac ccttacccag ttgttcaact cacccttcag attaaaaata actgaggtaa
     4081 gggcctgggt aggggaggtg gtgtgagacg ctcctgtctc tcctctgcat gccctgaggc
     4141 cctttgggga ggaggaatgt gcccaaggac taaaaaaagg ccatggagcc agaggggcga
     4201 gggcaacaga cctttcatgg gcaaaccttg gggcccgtag tgatcgattg acaagaactc
     4261 gccaatcgat acccttcttc ttctaacgga caggagggaa ctcgagttta ccactcccta
     4321 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     4381 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     4441 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     4501 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     4561 taccactccc tatcagtgat agagaaaagt gaaagtcgag ctcggtacca gcagaggact
     4621 ccaaatttag gcagcaggca tatgggatgg gatataaagg ggctggagca ctgagagctg
     4681 tcagagattt ctccaaccca ggtaagaggg agtttcgggt gggggctctt cacccacacc
     4741 agacctctcc ccacctagaa ggaaactgcc tttcctggaa gtggggttca ggccggtcag
     4801 agatctgaca gggtggcctt ccaccagcct gggaagttct cagtggcagg aggtttccac
     4861 aagaaacact ggatgcccct tcccttacgc tgtcttctcc atcttcctcc tggggatgct
     4921 cctccccgtc ttggtttatc ttggctcttc gtcttcagca agatttgccc tgtgctgtcc
     4981 actccatctt tctctactgt ctccgtgcct tgccttgcct tcttgcgtgt ccttcctttc
     5041 cacccatttc tcacttcacc ttttctcccc ttctcatttg tattcatcct tccttccttc
     5101 cttccttcct tccttccttc cttccttcct tcctttctcc cttccttcct tccttccttc
     5161 cttccttcct tccttccttc ctgtgtcaga gtgctgagaa tcacacctgg ggttcccacc
     5221 cttatgtaaa caatcttcca gtgagccaca gcttcagtgc tgctgggtgc tctcttacct
     5281 tcctcacccc ctggcttgtc ctgttccatc ctggtcagga tctctagatt ggtctcccag
     5341 cctctgctac tcctcttcct gcctgttcct ctctctgtcc agctgcgcca ctgtggtgcc
     5401 tcgttccagc tgtggtccac attcttcagg attctctgaa aagttaacca ggtgagaatg
     5461 tttcccctgt agacagcaga tcacgattct cccggaagtc aggcttccag ccctctcttt
     5521 ctctgcccag ctgcccggca ctcttagcaa acctcaggca cccttacccc acatagacct
     5581 ctgacagaga agcaggcact ttacatggag tcctggtggg agagccatag gctacggtgt
     5641 aaaagaggca gggaagtggt ggtgtaggaa agtcaggact tcacatagaa gcctagccca
     5701 caccagaaat gacagacaga tccctcctat ctcccccata agagtttgag tcgactgact
     5761 aactagaagc ttgatgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg
     5821 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg
     5881 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg
     5941 caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca
     6001 gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct
     6061 cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt
     6121 ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag
     6181 gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc
     6241 ttccctgtcc ttctgatttt aaaataacta taccagcagg aggacgtcca gacacagcat
     6301 aggctacctg ccatggccca accggtggga catttgagtt gcttgcttgg cactgtcctc
     6361 tcatgcgttg ggtccactca gtagatgcct gttgaattcc tgcagcccgg gggatccact
     6421 agttctagag cggccgttta aactagtata cacgtggcgc ccgggcggcc gc
//
