LOCUS       sequence-86568-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Wang D, Zhang Z, Chanda B, Jackson MB
  TITLE     Improved probes for hybrid voltage sensor imaging.
  JOURNAL   Biophys J. 2010 Oct 6;99(7):2355-65. doi: 10.1016/j.bpj.2010.07.037.
  PUBMED    20923671
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2010.07"; journalName: "Biophys J"; date: "2010-10-6-
            6"; volume: "99"; issue: "7"; pages: "2355-65"
COMMENT     Sequence Label: hVoS 1.5 thy-1
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(103..615,616..618,619..621))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     296..315
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     555..576
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cttggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
       61 ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat
      121 cttgaagttg gccttgatgc cgttcttctg cttgtcggcg gtgatataga cgttgtcgct
      181 gatggcgttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
      241 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
      301 gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc
      361 ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cgggcgaagc actgcacgcc
      421 ccaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat
      481 gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa
      541 cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag
      601 ctcctcgccc ttgctcacca ttcgaaaacc tctanctngc aanacttgcn acttgnaata
      661 tgganggcgg aaanntacac gacatccagc agganngaca gnngggtccc catttacctt
      721 gagggcttga gatccacgga gcacatgatg aatacctcag cccctctctt tatccccttc
      781 tcttagggag tgacactgcc taaggtcctt tgccactcag agaaagccaa ggaagtggga
      841 gcctatgtcc tgggtgtgtg tctgtgctgt ccctga
//
