LOCUS       sequence-86572-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Tong Q, Dalgin G, Xu H, Ting CN, Leiden JM, Hotamisligil GS
  TITLE     Function of GATA transcription factors in preadipocyte-adipocyte 
            transition.
  JOURNAL   Science 2000 Oct 6;290(5489):134-8.
  PUBMED    11021798
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2000-10-6"; volume: "290(5489)"; pages: "134-8"
COMMENT     Sequence Label: pcDNA-GATA3
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(687..705)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(688..705)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(723..739)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(723..739)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(736..758)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    747..763
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(771..777,778..795,796..801))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    816..837
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tgctgaccac accgacgccc atgcatccgc cctccggcct ctccttcgga cctcaccacc
       61 cttccagcat ggtcaccgcc atgggttaga gaggcagagc cctgctccac atgcgtgagg
      121 agtctccaag tgtgcgaaga gttcctccga ccccttctac ttgcgttttt cgcaggagca
      181 gtatcatgaa gcccgaaagc gacagatctg tgtttttgaa ggcagaaagc aaaatgtttg
      241 cttctttttt caaaggagct cgaggtggtg tctgcattcc aaccactgaa tccggatccc
      301 atttgtgaat aagccattca gactcatatt ccctatttaa cagggtctct agtgctgtga
      361 aaaaaatatt gctgaacatt gcatataact tatattgtaa gaaatactgt acatttgagg
      421 aagactttat tgtacctgga tagctgtaag aaaggcatga aggacgccaa gagttttaag
      481 gaatataggg ggattaaagt atggagatac agaagaaacc actaagtctg atgtccaaat
      541 gggcacactg tcagttttgt ttcccttcag ttgtttgatg catttaaaaa aaaaaaaaag
      601 aaagaaggaa ttcggtaccc cgggttcgaa atcgataagc ttggatccgg agagctccca
      661 acgcgttgga tgcatagctt gagtattcta tagtgtcacc taaatagctt ggcgtaatca
      721 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
      781 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
      841 gcgttgcgct cactgcccgc ttttccagtc gggaaacctg tcgtgccagc tgcattaatg
      901 aatcggccaa cgcgcg
//
