LOCUS       sequence-86573-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a plant arginyl-tRNA synthetase in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Igloi GL, Schiefermayr E
  TITLE     Amino acid discrimination by arginyl-tRNA synthetases as revealed by
            an examination of natural specificity variants.
  JOURNAL   FEBS J. 2009 Mar;276(5):1307-18. doi: 
            10.1111/j.1742-4658.2009.06866.x.
  PUBMED    19187230
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1742-4658.2009.06866"; journalName: "FEBS J"; date: 
            "2009-03"; volume: "276"; issue: "5"; pages: "1307-18"
COMMENT     Sequence Label: pET-Gm1
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    76..100
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             115..137
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             145..471
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             493..510
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             520..537
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             544..588
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             604..618
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatcgat ctcgatcccg cgaaattaat
       61 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt
      121 taactttaag aaggagatat acatatgagc gataaaatta ttcacctgac tgacgacagt
      181 tttgacacgg atgtactcaa agcggacggg gcgatcctcg tcgatttctg ggcagagtgg
      241 tgcggtccgt gcaaaatgat cgccccgatt ctggatgaaa tcgctgacga atatcagggc
      301 aaactgaccg ttgcaaaact gaacatcgat caaaaccctg gcactgcgcc gaaatatggc
      361 atccgtggta tcccgactct gctgctgttc aaaaacggtg aagtggcggc aaccaaagtg
      421 ggtgcactgt ctaaaggtca gttgaaagag ttcctcgacg ctaacctggc cggttctggt
      481 tctggccata tgcaccatca tcatcatcat tcttctggtc tggtgccacg cggttctggt
      541 atgaaagaaa ccgctgctgc taaattcgaa cgccagcaca tggacagccc agatctgggt
      601 accgacgacg acgacaaggc catggcgtat gttgaagtat attgtccagc tagtgttaag
      661 aagcagttgg cgaaggagtt tgaggagtct ctccggataa ccgtgcctga tgaacccgat
      721 gttgtgcctt ctattgatcc ttgcgctgcc aacaaagctg gtattaaatt cgctgattat
      781 caatgtaata atgcaatggg tctatttgct aagatgaaag ggaagcagac aggattcagg
      841 ggtccccagg cagttggaca ggccatactt aaccatcttc cgcagtctga aatgatagaa
      901 tcatgctctg tagctggtcc tgggtttgtg aatattgt
//
