LOCUS       sequence-8658-ex        2460 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   McKinnell IW, Ishibashi J, Le Grand F, Punch VG, Addicks GC, 
            Greenblatt JF, Dilworth FJ, Rudnicki MA
  TITLE     Pax7 activates myogenic genes by recruitment of a histone 
            methyltransferase complex.
  JOURNAL   Nat Cell Biol. 2008 Jan . 10(1):77-84.
  PUBMED    18066051
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2008 Jan . 10(1):77-84."
COMMENT     Sequence Label: pBRIT HA/FLAG
FEATURES             Location/Qualifiers
     source          1..2460
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             331..747
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     677..699
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     715..731
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    758..791
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        798..816
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             889..915
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             926..997
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    1015..1048
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(1060..1080)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1065..1394
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1245..1380
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1404..2003
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1404..1423)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1900..1920
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     gap             2004..2052
                     /estimated_length=49
ORIGIN
        1 tgcgtctgta ctagtagcta ctagctcgta tctggcggac ccgtggtgga actgacgagt
       61 tctgaacanc gggcgcaacc ntgggagacg tcccagggac tttggggccg tttttgtggc
      121 ccgacctgag gaagggagtc gatgtggaat ccgaccccgt caggatatgt ggttctggta
      181 ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc ggtttggaac
      241 cgaagccgcg cgtcttgtct gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg
      301 actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc cttaagtttg
      361 accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt agatgtcaag
      421 aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt cggatggccg
      481 cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt cttttcacct
      541 ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc cttggctttt
      601 gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc tcttcctcca
      661 tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc ctccctttat
      721 ccagccctca ctccttctct aggcgccggc cggatctata acttcgtata atgtatgcta
      781 tacgaagtta tggatctaat taaccctcac taaagggaac aaaagctgga gctccaccgc
      841 ggtggcggcc gctctagccc gggcggatcc gaattcgcat gcgtcgacta tccatacgac
      901 gtgcctgact acgcctgaac tcgaggacta caaggatgac gatgacaagg attacaaaga
      961 cgacgatgat aaggactata aggatgatga cgacaaataa tagtctagag atccataact
     1021 tcgtataatg tatgctatac gaagttatct cgaccctgtg gaatgtgtgt cagttagggt
     1081 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     1141 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     1201 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     1261 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     1321 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     1381 taggcttttg caaaaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     1441 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     1501 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     1561 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     1621 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     1681 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     1741 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     1801 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     1861 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     1921 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     1981 tgacccgcaa gcccggtgcc tgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat
     2101 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
     2161 gaaaaaatac ataactgaga atagagaagt tcagatcagg tcaggaacag atggaacagc
     2221 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgcccggctc agggccaaga
     2281 acagatggaa cagctgaata tgggccaaca gaatatctgt ggtaagcagt tcctgccccg
     2341 gctcagggcc aagaacagat ggtccccaaa tgcggtccag ccctcagcag tttctagaga
     2401 acatcagatg tttccagggt gcccaagact gaatgacctg tgctatgact acatcagagc
//
