LOCUS       sequence-86581-e        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a plant arginyl-tRNA synthetase in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Igloi GL, Schiefermayr E
  TITLE     Amino acid discrimination by arginyl-tRNA synthetases as revealed by
            an examination of natural specificity variants.
  JOURNAL   FEBS J. 2009 Mar;276(5):1307-18. doi: 
            10.1111/j.1742-4658.2009.06866.x.
  PUBMED    19187230
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1742-4658.2009.06866"; journalName: "FEBS J"; date: 
            "2009-03"; volume: "276"; issue: "5"; pages: "1307-18"
COMMENT     Sequence Label: pET-Gm1
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tcggatccga tatcagccat ggtcatagcc
      121 tgtatacagg ttcaattcca aggagataaa agcattgtct catcacagtc actgttgctt
      181 cacatagcaa gagtctacta gtctcctcag gcgaccccac aacctggcaa ttagtataaa
      241 atttttttgt aaagatttct gtcaaattat agaggtattc acacagcaca ttgggcaaca
      301 aattggtcaa cgactcctca aaaacctcag gaaattgtat caaatgaagc cccaatgcac
      361 gttcatcttc atgattcgac actatattcc catttttctt tatttcttcc atatctttac
      421 cagatttcct catgatagaa cagatcctag catgcgcata ctgcaaataa acagcagtat
      481 ttcccttatc attaagcatc tgatcaaagc tgaatgtgta attcgttaat ctgttgatcc
      541 tcaagtcagc atacttaaca gctccgtaac caactgcttc tgctgtcttt tcgatctctt
      601 cctcagacca atctttaaca gcatcacgct cgaggaggga agctttacaa cgccttttgg
      661 cttcatcaag caactcaacc aatctaacag tttcactgct gcgtgtccga aatcgttttc
      721 catcttcccc aagaacaaga ccaaaaccaa tatgagtaca tttggggaac tcattttcat
      781 gatttggcag ccatcctgca cgcctaaagg ccttaaaaac catatcaaag tgttgccact
      841 gcccaacatc tgtaacgtat atattccact caacattttc cacatttaga cgattccaaa
      901 gtgctgctag atcagttgaa gaatagttgt agccaccatc tcttttcaca gcaataattg
      961 gtatatttac accctcaaca aatatcacac gagcaccatc atcttcttca atcagtcc
//
