LOCUS       sequence-86582-e         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a plant arginyl-tRNA synthetase in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Hogg J, Schiefermayr E, Schiltz E, Igloi GL
  TITLE     Expression and properties of arginyl-tRNA synthetase from jack bean 
            (Canavalia ensiformis).
  JOURNAL   Protein Expr Purif. 2008 Oct;61(2):163-7. doi: 
            10.1016/j.pep.2008.05.008. Epub 2008 May 23.
  PUBMED    18562210
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.pep.2008.05.008"; journalName: "Protein Expr Purif"; 
            date: "2008-10"; volume: "61"; issue: "2"; pages: "163-7"
COMMENT     Sequence Label: pET-Ce25
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..24
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5..23
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    24..48
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             63..85
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             93..419
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             441..458
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             468..485
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             492..536
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             552..566
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata
       61 attttgttta actttaagaa ggagatatac atatgagcga taaaattatt cacctgactg
      121 acgacagttt tgacacggat gtactcaaag cggacggggc gatcctcgtc gatttctggg
      181 cagagtggtg cggtccgtgc aaaatgatcg ccccgattct ggatgaaatc gctgacgaat
      241 atcagggcaa actgaccgtt gcaaaactga acatcgatca aaaccctggc actgcgccga
      301 aatatggcat ccgtggtatc ccgactctgc tgctgttcaa aaacggtgaa gtggcggcaa
      361 ccaaagtggg tgcactgtct aaaggtcagt tgaaagagtt cctcgacgct aacctggccg
      421 gttctggttc tggccatatg caccatcatc atcatcattc ttctggtctg gtgccacgcg
      481 gttctggtat gaaagaaacc gctgctgcta aattcgaacg ccagcacatg gacagcccag
      541 atctgggtac cgacgacgac gacaaggcca tggtttttat tgaactggat tgtccggcca
      601 gtgttaaaaa acagctggca aagctgtttg aggtgtctct ga
//
