LOCUS       sequence-86585-e        1096 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a plant arginyl-tRNA synthetase in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Hogg J, Schiefermayr E, Schiltz E, Igloi GL
  TITLE     Expression and properties of arginyl-tRNA synthetase from jack bean 
            (Canavalia ensiformis).
  JOURNAL   Protein Expr Purif. 2008 Oct;61(2):163-7. doi: 
            10.1016/j.pep.2008.05.008. Epub 2008 May 23.
  PUBMED    18562210
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.pep.2008.05.008"; journalName: "Protein Expr Purif"; 
            date: "2008-10"; volume: "61"; issue: "2"; pages: "163-7"
COMMENT     Sequence Label: pET-Ce25
FEATURES             Location/Qualifiers
     source          1..1096
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atccgatatc agccatggtc atagcttgta
      121 tacaggatca atcccaagga gataaaagca ctttcgcatc acagttgccg ttgcttcaca
      181 tagtaagagt ctactagttt gctcaggcga cccaatgacc tgacaattag tataaaactt
      241 tttggtaaag atttctgtca aattatagag gtattcacac aacacattgg gtaataaatt
      301 ggccaatgac tccacaaaga cctcaggaaa ttgtatcaag tgaagcccca atgcacgctc
      361 gtcttcatga ttcaatacta tattcccatt tttctttaat tcttctatat ctttaccaga
      421 tttcctgata atggaacaga tcctagcatg tgcatatagc aaataaacag cagtgttccc
      481 cttatcatta agcatctgat cgaagttgaa tgtgtagttt gttaatctgt tgattttcaa
      541 gtcagcatac ttaacagccc cataaccaat tgcctctgat gttttttcaa tctcttcttc
      601 agaccaatct ttagcagcgt cacgttcaag cagggcagat ttacaacgcc ttttggcttc
      661 gtctagtaga tcaaccaatc gaacagtttc gctgctgcga ctccgaaatc gtttcccatc
      721 ttccccaaga acaagaccaa aaccaatatg ggtacatttt gggaagtcat tttcatcctt
      781 tggcagccat cctgcacgcc tgaaggcctt aaaaagcata tcaaagtgct gccactgccc
      841 aacatctgta acataaatgt tccactcaag tttttccaca tttaggcgat accaaagtga
      901 cgctagatca gatgaaaaat agttgtagcc accatctctt ttcacagcaa taattggtat
      961 gtttacaccc tcaacaaata tcacacgagc accatcactg tcttcaatca gtcctaattt
     1021 atccaacttc tccaaagttg gagggattaa atcattaaag aaactctctc ccattgcctc
     1081 tactcgaact ccaagc
//
