LOCUS       sequence-86587-e        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ETS2A-c061
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             603..620
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(673..691)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      687..734
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(858..877)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgaat
      121 aagccaacca tgtctttcaa ggattacatc caagagagga gtgacccggt ggagcaaggc
      181 aaaccagtta tacctgcagc tgtgctggcc ggcttcacag gaagtggacc tattcagctg
      241 tggcagtttc tcctggagct gctatcagac aaatcctgcc agtcattcat cagctggact
      301 ggagacggat gggagtttaa gctcgccgac cccgatgagg tggcccgccg gtggggaaag
      361 aggaaaaata agcccaagat gaactacgag aagctgagcc ggggcttacg ctactattac
      421 gacaagaaca tcatccacaa gacgtcgggg aagcgctacg tgtaccgctt cgtgtgcgac
      481 ctccagaact tgctggggtt cacgcccgag gaactgcacg ccatcctggg cgtccagtga
      541 cagtaaaggt ggatacggat ccgaattcga gctccgtcga caagcttgcg gccgcactcg
      601 agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt
      661 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
      721 tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg
      781 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
      841 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
      901 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
      961 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgta
//
