LOCUS       sequence-86588-e        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BRPF1B-c001
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             525..542
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(595..613)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      609..656
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(780..799)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatggag
      121 atgcagctga ctcctttcct catcctcctt cgcaaaacct tggagcagct ccaagagaag
      181 gacacaggca acatcttcag cgagccggtc cctctgtctg aggtacctga ctacctagac
      241 cacatcaaaa agcccatgga ctttttcacc atgaagcaga acttggaggc ttaccgctac
      301 ctgaattttg atgattttga ggaggacttc aacctcatcg tcagcaactg cctcaagtat
      361 aacgccaagg acaccatctt ctaccgggca gcagtgcggc ttcgtgagca gggtggtgct
      421 gtgctccgcc aggcccggcg ccaggcagaa aaaatgggct gacagtaaag gtggatacgg
      481 atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc
      541 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      601 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      661 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
      721 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
      781 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
      841 aaatcggggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
      901 aacttgatta gggtgatggt tcacgtagtg gggccatcgc cctgatagac ggtttttcgc
      961 cctttgacgt tggagtccac gtttctttta atagtggact cttgtttcca aactggaaca
     1021 acactcaacc c
//
