LOCUS       sequence-8659-ex        3926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   McKinnell IW, Ishibashi J, Le Grand F, Punch VG, Addicks GC, 
            Greenblatt JF, Dilworth FJ, Rudnicki MA
  TITLE     Pax7 activates myogenic genes by recruitment of a histone 
            methyltransferase complex.
  JOURNAL   Nat Cell Biol. 2008 Jan . 10(1):77-84.
  PUBMED    18066051
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2008 Jan . 10(1):77-84."
COMMENT     Sequence Label: pBRIT Pax7 FLAG
FEATURES             Location/Qualifiers
     source          1..3926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             331..747
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     677..699
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     715..731
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    758..791
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        798..816
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      871..880
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      875..884
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2392..2463
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    2481..2514
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2526..2546)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2531..2860
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2711..2846
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2870..3469
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2870..2889)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3366..3386
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     gap             3470..3518
                     /estimated_length=49
ORIGIN
        1 tgcgtctgta ctagtagcta ctagctcgta tctggcggac ccgtggtgga actgacgagt
       61 tctgaacanc gggcgcaacc ntgggagacg tcccagggac tttggggccg tttttgtggc
      121 ccgacctgag gaagggagtc gatgtggaat ccgaccccgt caggatatgt ggttctggta
      181 ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc ggtttggaac
      241 cgaagccgcg cgtcttgtct gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg
      301 actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc cttaagtttg
      361 accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt agatgtcaag
      421 aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt cggatggccg
      481 cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt cttttcacct
      541 ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc cttggctttt
      601 gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc tcttcctcca
      661 tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc ctccctttat
      721 ccagccctca ctccttctct aggcgccggc cggatctata acttcgtata atgtatgcta
      781 tacgaagtta tggatctaat taaccctcac taaagggaac aaaagctgga gctccaccgc
      841 ggtggcggcc gctctagccc gggcggatcc gccaccatgg cggcgctgcc cggcgcggtc
      901 cccaggatga tgagacccgg cccggggcag aactacccgc gcaccggctt ccccctggaa
      961 gtgtccaccc ctcttggcca aggccgggtc aatcagcttg gtggggtctt catcaatggt
     1021 cgacccctgc cgaaccacat ccgtcacaag atagtggaaa tggcccacca tggcatccgg
     1081 ccctgcgtca tctcccgtca gctccgtgtt tctcatggtt gtgtctccaa gattctgtgc
     1141 cgatatcagg agactgggtc catccggccc ggggctatcg gaggcagcaa gcccagacag
     1201 gtggcgactc cggatgtgga gaaaaagatt gaggagtata agagagagaa ccccgggatg
     1261 ttcagctggg aaatccggga ccggctgctg aaggacggtc actgcgaccg aagcacggtg
     1321 ccctcagtga gttcgattag ccgagtgctc agaatcaagt tcgggaagaa agaggacgac
     1381 gaggaaggag acaagaaaga agaagatggc gagaagaaag ccaaacacag catcgatggc
     1441 atcctgggcg acaaagggaa ccgtctggat gagggctcag atgtggaatc agaacccgac
     1501 ctccccctga agcgcaagca gcgccgcagt cggaccacgt tcacagccga gcagctggag
     1561 gagctagaga aggcctttga gaggacccac tacccggaca tctacacccg ggaggagctg
     1621 gcacagagga ccaagctcac ggaggcacgc gtccaggtct ggttcagtaa ccggcgtgcc
     1681 cgctggcgca agcaggcagg agctaaccag ctggccgcct tcaaccacct tctgccggga
     1741 ggtttcccac ccaccggcat gcccacgctg ccaccctacc agctgccgga ctctacctac
     1801 cccaccacca ccatctccca agatgggggc agcacagtac acaggcccca gccccttccg
     1861 ccatcaacca tgcatcaggg tgggctggct gcggccgctg cagcagcaga caccagctct
     1921 gcctacggag cccgccacag cttctccagc tactctgaca gcttcatgaa ccctggggct
     1981 ccctccaacc acatgaaccc tgtcagcaat ggcctgtctc ctcaggtcat gagcatcctt
     2041 agcaacccga gtgccgtgcc tccacagccc caggccgact tctccatctc cccgctgcat
     2101 ggaggcctgg actcggcttc ctccatctca gccagctgca gccaacgggc cgactccatc
     2161 aagccaggag acagcttgcc cacgtcccag tcttactgcc cacccaccta cagcaccact
     2221 ggctacagtg tggaccctgt ggctggctac cagtacagcc agtatggcca aactgctgtt
     2281 gattacctgg ccaaaaacgt gagcctgtcc acacagcgcc gtatgaagct tggggaacac
     2341 tccgctgtgc tgggacttct tcctgtggaa acgggacaag cctacctcga ggactacaag
     2401 gatgacgatg acaaggatta caaagacgac gatgataagg actataagga tgatgacgac
     2461 aaataatagt ctagagatcc ataacttcgt ataatgtatg ctatacgaag ttatctcgac
     2521 cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     2581 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     2641 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     2701 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     2761 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     2821 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttacca tgaccgagta
     2881 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     2941 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     3001 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     3061 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     3121 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     3181 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     3241 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     3301 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     3361 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     3421 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgan nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat cgataaaata aaagatttta
     3541 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct
     3601 taagtaacgc cattttgcaa ggcatggaaa aaatacataa ctgagaatag agaagttcag
     3661 atcaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc
     3721 agttcctgcc cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaacagaat
     3781 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccaaatgcg
     3841 gtccagccct cagcagtttc tagagaacat cagatgtttc cagggtgccc aagactgaat
     3901 gacctgtgct atgactacat cagagc
//
