LOCUS       sequence-86591-e        1075 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1075)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BAZ2AA-c002
FEATURES             Location/Qualifiers
     source          1..1075
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..108
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             489..506
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(559..577)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      573..620
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      657..1015
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(744..763)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     954..975
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat
       61 cattcttctg gtgtagatct gggtaccgag aacctgtact tccaatccat gcactccgat
      121 ctgacgtttt gcgaaattat cctgatggaa atggaaagcc atgacgcagc ttggccgttc
      181 ctggaaccgg ttaacccgcg cctggtcagc ggctatcgcc gtatcatcaa aaacccgatg
      241 gatttctcta cgatgcgcga acgtctgctg cgtggcggtt acaccagctc tgaagaattc
      301 gcggccgatg ccctgctggt gtttgacaac tgtcagacct tcaatgaaga tgacagcgaa
      361 gttggcaaag cgggtcacat catgcgtcgc tttttcgaat ctcgctggga agaattttat
      421 cagtgacagt aaaggtggat acggatccga attcgagctc cgtcgacaag cttgcggccg
      481 cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag
      541 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
      601 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc
      661 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
      721 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
      781 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
      841 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
      901 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
      961 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     1021 atttgccaat tcggcctatt ggttaaaaat gagctgatta caaaatttaa cgcga
//
