LOCUS       sequence-86592-e        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PCBD2A-c103
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..113
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             488..505
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(558..576)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      572..619
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      656..1014
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(743..762)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt tagaaggaga tatacatatg caccatcatc
       61 atcatcattc ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatgtcat
      121 ctggcaccca tcgtctgacg gctgaagaac gcaaccaggc gattctggat ctgaaagcgg
      181 ccggttggag cgaactgtct gaacgtgacg ccatctacaa ggaatttagt ttccacaact
      241 tcaaccaggc atttggcttc atgtcccgcg ttgccctgca agcagaaaaa atgaatcatc
      301 acccggaatg gttcaacgtg tacaataagg ttcaaattac cctgacgtca catgattgcg
      361 gtgaactgac caaaaaggac gtcaaactgg cgaagtttat cgaaaaagca gctgcgtcgg
      421 tgtgacagta aaggtggata cggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc
      481 actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc
      541 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
      601 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg
      661 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
      721 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
      781 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
      841 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
      901 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
      961 ttgttcccaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     1021 a
//
