LOCUS       sequence-86594-e         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BRD2A-c014
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..113
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             506..523
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(576..594)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      590..637
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(761..780)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt tagaaggaga tatacatatg caccatcatc
       61 atcatcattc ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatggaac
      121 aactgaagca ttgcaatggc attcttaaag agctcctgag taaaaaacac gccgcctatg
      181 cgtggccatt ttataaaccg gtggatgcct ctgcgctggg tctgcatgat tatcacgata
      241 tcattaaaca tccgatggat ctctcaaccg ttaaacgtaa aatggaaaat cgcgattatc
      301 gtgatgccca ggaatttgcg gcggatgtac gcctcatgtt ttcgaactgc tacaaatata
      361 accctccaga tcacgatgtt gtggcaatgg cacgaaagct acaggatgta tttgagttcc
      421 gttatgccaa gatgccagat tgacagtaaa ggtggatacg gatccgaatt cgagctccgt
      481 cgacaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa
      541 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
      601 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
      661 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
      721 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
      781 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
      841 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
      901 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
      961 ttctttaata gtggactctg
//
