LOCUS       sequence-86597-e        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA tagged human MEK1 C121S from puromycin resistance 
            retroviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pBABEpuro-HA-MEK1 C121S
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..56
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ggccggatcc gaatatcgcc gccaccatgt acccatacga tgttccagat tacgctccca
       61 agaagaagcc gacgcccatc cagctgaacc cggcccccga cggctctgca gttaacggga
      121 ccagctctgc ggagaccaac ttggaggcct tgcagaagaa gctggaggag ctagagcttg
      181 atgagcagca gcgaaagcgc cttgaggcct ttcttaccca gaagcagaag gtgggagaac
      241 tgaaggatga cgactttgag aagatcagtg agctgggggc tggcaatggc ggtgtggtgt
      301 tcaaggtctc ccacaagcct tctggcctgg tcatggccag aaagctaatt catctggaga
      361 tcaaacccgc aatccggaac cagatcataa gggagctgca ggttctgcat gagtccaact
      421 ctccgtacat cgtgggcttc tatggtgcgt tctacagcga tggcgagatc agtatctgca
      481 tggagcacat ggatggaggt tctctggatc aagtcctgaa gaaagctgga agaattcctg
      541 aacaaatttt aggaaaagtt agcattgctg taataaaagg cctgacatat ctgagggaga
      601 agcacaagat catgcacaga gatgtcaagc cctccaacat cctagtcaac tcccgtgggg
      661 agatcaagct ctgtgacttt ggggtcagcg ggcagctcat cgactccatg gccaactcct
      721 tcgtgggcac aaggtcctac atgtcgccag aaagactcca ggggactcat taccctgtgc
      781 agtcagacat ctggagcatg ggactgtctc tggtagagat ggcggttggg aggtatccca
      841 tccctcctcc agatgccaag gagctggagc tgatgtttgg gtgccaggtg gaaggagatg
      901 cggctgagac cccacccagg ccaaggaccc ccgggaggcc ccttagctca tacggaatgg
      961 acagccgacc tcccatggca atttttgagt tgttggatta catagtcaac gagcctcctc
     1021 caaaactgcc cagtggagtg ttcagtctgg aatttca
//
