LOCUS       sequence-86598-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express Cup9p‐FLAG from the PGAL1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Byrd C, Turner GC, Varshavsky A
  TITLE     The N-end rule pathway controls the import of peptides through 
            degradation of a transcriptional repressor.
  JOURNAL   EMBO J. 1998 Jan 2. 17(1):269-77.
  PUBMED    9427760
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            1998 Jan 2. 17(1):269-77."
COMMENT     Sequence Label: pCB116
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(33..280)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     246..264
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     282..298
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     283..299
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(323..346)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgactcacta tagggcgaat tgggtaccgg ccgcaaatta aagccttcga gcgtcccaaa
       61 accttctcaa gcaaggtttt cagtataatg ttacatgcgt acacgcgtct gtacagaaaa
      121 aaaagaaaaa tttgaaatat aaataacgtt cttaatacta acataactat aaaaaaataa
      181 atagggacct agacttcagg ttgtctaact ccttcctttt cggttagagc ggatgtgggg
      241 ggagggcgtg aatgtaagcg tgacataact aattacatga ctcgaggtcg acggtatcga
      301 taagcttgat atcgaattct catttatcat catcgtcttt gtaatcattc atatcagggt
      361 tggatagctt tttcaattct tccagcctat caattagttt tttacggcgt gtaacggggg
      421 catgagcaaa tctcttggat aattcataat ccgtggagca ggttgacgta gcatcgtaca
      481 tagtattatt tgtggcagat aatgtgttat gatatgctga gacgttttga actcgttcca
      541 ctggggtatt attcgcgttg tcattaggaa ttgagtttac cagggtatag taatcactaa
      601 atattttgcg tcttctcaca tttatgaacc aattagaaag ttgaatcttg gttagcccag
      661 ttttgattaa caactccctc ttttcttgtt gagttgggta ggggttattc aggtggttca
      721 gcaaccaagt attcagtatc tgcactgttt cctttggcag gttagacctc ctgcccgaat
      781 tttgcttcct ccttgattta gattctacaa aatccgaaaa tggcctgttg tattgtgccc
      841 ccgcatcctt ttccttattg attatttcat atattggggc gga
//
