LOCUS       sequence-86599-e        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GAKA-c028
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             9..31
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             42..71
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             84..104
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat catcatcatc
       61 accatcatca cgatctgggt accgagaacc tgtacttcca atccatgcca ggctccctgg
      121 gcggtgcttc cggccgcgac cagagtgact tcgtggggca gacggtggaa ctgggcgagc
      181 tgcggctgcg ggtgcggcgg gtcctggccg aaggagggtt tgcatttgtg tatgaagctc
      241 aagatgtggg gagtggcaga gagtatgcat taaagaggct attatccaat gaagaggaaa
      301 agaacagagc catcattcaa gaagtttgct tcatgaaaaa gctttccggc cacccgaaca
      361 ttgtccagtt ttgttctgca gcgtctatag gaaaagagga gtcagacacg gggcaggctg
      421 agttcctctt gctcacagag ctctgtaaag ggcagctggt ggaatttttg aagaaaatgg
      481 aatctcgagg ccccctttcg tgcgacacgg ttctgaagat cttctaccag acgtgccgcg
      541 ccgtgcagca catgcaccgg cagaagccgc ccatcatcca cagggacctc aaggttgaga
      601 acttgttgct tagtaaccaa gggaccatta agctgtgtga ctttggcagt gccacgacca
      661 tctcgcacta ccctgactac agctggagcg cccagaggcg agccctggtg gaggaagaga
      721 tcacgaggaa tacaacacca atgtatagaa caccagaaat catagacttg tattccaact
      781 tcccgatcgg cgagaagcag gatatctggg ccctgggctg catcttgtac ctgctgtgct
      841 tccggcagca cccttttgag gatggagcga aacttcgaat agtcaatggg aagtactcga
      901 tccccccgca cgacacgcag tacacggtct tccacagcct catccgcgcc atgctgcagg
      961 tgaacccgga ggagcggctg tccatcgccg aggtggtgca ccagctgcag gagatcgcgg
     1021 ccgcccgcaa cgtgaacccc aagtctccca tcacagagct cctgga
//
