LOCUS       sequence-8660-ex        3830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   McKinnell IW, Ishibashi J, Le Grand F, Punch VG, Addicks GC, 
            Greenblatt JF, Dilworth FJ, Rudnicki MA
  TITLE     Pax7 activates myogenic genes by recruitment of a histone 
            methyltransferase complex.
  JOURNAL   Nat Cell Biol. 2008 Jan . 10(1):77-84.
  PUBMED    18066051
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2008 Jan . 10(1):77-84."
COMMENT     Sequence Label: pBRIT Pax7 34C
FEATURES             Location/Qualifiers
     source          1..3830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             331..747
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     677..699
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     715..731
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    758..791
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        798..816
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      871..880
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2296..2367
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    2385..2418
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2430..2450)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2435..2764
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2615..2750
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2774..3373
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2774..2793)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3270..3290
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     gap             3374..3422
                     /estimated_length=49
ORIGIN
        1 tgcgtctgta ctagtagcta ctagctcgta tctggcggac ccgtggtgga actgacgagt
       61 tctgaacanc gggcgcaacc ntgggagacg tcccagggac tttggggccg tttttgtggc
      121 ccgacctgag gaagggagtc gatgtggaat ccgaccccgt caggatatgt ggttctggta
      181 ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc ggtttggaac
      241 cgaagccgcg cgtcttgtct gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg
      301 actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc cttaagtttg
      361 accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt agatgtcaag
      421 aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt cggatggccg
      481 cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt cttttcacct
      541 ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc cttggctttt
      601 gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc tcttcctcca
      661 tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc ctccctttat
      721 ccagccctca ctccttctct aggcgccggc cggatctata acttcgtata atgtatgcta
      781 tacgaagtta tggatctaat taaccctcac taaagggaac aaaagctgga gctccaccgc
      841 ggtggcggcc gctctagccc gggcggatcc gccaccatgg gccaaggccg ggtcaatcag
      901 cttggtgggg tcttcatcaa tggtcgaccc ctgccgaacc acatccgtca caagatagtg
      961 gaaatggccc accatggcat ccggccctgc gtcatctccc gtcagctccg tgtttctcat
     1021 ggttgtgtct ccaagattct gtgccgatat caggagactg ggtccatccg gcccggggct
     1081 atcggaggca gcaagcccag acaggtggcg actccggatg tggagaaaaa gattgaggag
     1141 tataagagag agaaccccgg gatgttcagc tgggaaatcc gggaccggct gctgaaggac
     1201 ggtcactgcg accgaagcac ggtgccctca gtgagttcga ttagccgagt gctcagaatc
     1261 aagttcggga agaaagagga cgacgaggaa ggagacaaga aagaagaaga tggcgagaag
     1321 aaagccaaac acagcatcga tggcatcctg ggcgacaaag ggaaccgtct ggatgagggc
     1381 tcagatgtgg aatcagaacc cgacctcccc ctgaagcgca agcagcgccg cagtcggacc
     1441 acgttcacag ccgagcagct ggaggagcta gagaaggcct ttgagaggac ccactacccg
     1501 gacatctaca cccgggagga gctggcacag aggaccaagc tcacggaggc acgcgtccag
     1561 gtctggttca gtaaccggcg tgcccgctgg cgcaagcagg caggagctaa ccagctggcc
     1621 gccttcaacc accttctgcc gggaggtttc ccacccaccg gcatgcccac gctgccaccc
     1681 taccagctgc cggactctac ctaccccacc accaccatct cccaagatgg gggcagcaca
     1741 gtacacaggc cccagcccct tccgccatca accatgcatc agggtgggct ggctgcggcc
     1801 gctgcagcag cagacaccag ctctgcctac ggagcccgcc acagcttctc cagctactct
     1861 gacagcttca tgaaccctgg ggctccctcc aaccacatga accctgtcag caatggcctg
     1921 tctcctcagg tcatgagcat ccttagcaac ccgagtgccg tgcctccaca gccccaggcc
     1981 gacttctcca tctccccgct gcatggaggc ctggactcgg cttcctccat ctcagccagc
     2041 tgcagccaac gggccgactc catcaagcca ggagacagct tgcccacgtc ccagtcttac
     2101 tgcccaccca cctacagcac cactggctac agtgtggacc ctgtggctgg ctaccagtac
     2161 agccagtatg gccaaactgc tgttgattac ctggccaaaa acgtgagcct gtccacacag
     2221 cgccgtatga agcttgggga acactccgct gtgctgggac ttcttcctgt ggaaacggga
     2281 caagcctacc tcgaggacta caaggatgac gatgacaagg attacaaaga cgacgatgat
     2341 aaggactata aggatgatga cgacaaataa tagtctagag atccataact tcgtataatg
     2401 tatgctatac gaagttatct cgaccctgtg gaatgtgtgt cagttagggt gtggaaagtc
     2461 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     2521 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     2581 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     2641 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     2701 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     2761 caaaaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     2821 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     2881 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     2941 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     3001 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     3061 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     3121 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     3181 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     3241 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     3301 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     3361 gcccggtgcc tgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
     3481 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaaatac
     3541 ataactgaga atagagaagt tcagatcagg tcaggaacag atggaacagc tgaatatggg
     3601 ccaaacagga tatctgtggt aagcagttcc tgcccggctc agggccaaga acagatggaa
     3661 cagctgaata tgggccaaca gaatatctgt ggtaagcagt tcctgccccg gctcagggcc
     3721 aagaacagat ggtccccaaa tgcggtccag ccctcagcag tttctagaga acatcagatg
     3781 tttccagggt gcccaagact gaatgacctg tgctatgact acatcagagc
//
