LOCUS       sequence-86602-e         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BLMA-c800
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct
       61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatgtccag aaatctgaaa
      121 catgagcgtt tccaaagtct tagttttcct catacaaagg aaatgatgaa gatttttcat
      181 aaaaaatttg gcctgcataa ttttagaact aatcagctag aggcgatcaa tgctgcactg
      241 cttggtgaag actgttttat cctgatgccg actggaggtg gtaagagttt gtgttaccag
      301 ctccctgcct gtgtttctcc tggggtcact gttgtcattt ctcccttgag atcacttatc
      361 gtagatcaag tccaaaagct gacttccttg gatattccag ctacatatct gacaggtgat
      421 aagactgact cagaagctac aaatatttac ctccagttat caaaaaaaga cccaatcata
      481 aaacttctat atgtcactcc agaaaagatc tgtgcaagta acagactcat ttctactctg
      541 gagaatctct atgagaggaa gctcttggca cgttttgtta ttgatgaagc acattgtgtc
      601 agtcagtggg gacatgattt tcgtcaagat tacaaaagaa tgaatatgct tcgccagaag
      661 tttccttctg ttccggtgat ggctcttacg gccacagcta atcccagggt acagaaggac
      721 atcctgactc agctgaagat tctcagacct caggtgttta gcatgagctt taacagacat
      781 aatctgaaat actatgtatt accgaaaaag cctaaaaagg tggcatttga ttgcctagaa
      841 tggatcagaa agcaccaccc atatgattca gggataattt actgcctctc caggcgagaa
      901 tgtgacacca tggctgacac gttacagaga gatgggctcg ctgctcttgc ttaccatgct
      961 ggcctcagtg attctgccag agatgaagtg ca
//
