LOCUS       sequence-86604-e        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PRKCBP1A-c092
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             63..233
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     CDS             240..260
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtggataa caagttcaac aaggagcgtc gaagagctcg ccgtgaaatt cgccatctgc
      121 cgaacctgaa ccgcgaacag cgtcgcgcat ttattcgcag cctgcgcgat gatccgagcc
      181 agagcgcgaa cctgctggcg gaagcgaaga agctgaacga tgcgcagccg aagggtaccg
      241 agaacctgta cttccaatcc atgcaggatg gacggaatga tttctactgc tgggtttgtc
      301 accgggaagg ccaagtcctt tgctgtgagc tctgtccccg ggtttatcac gctaagtgtc
      361 tgagactgac atcggaacca gagggggact ggttttgtcc tgaatgtgag aaaattacag
      421 tagcagaatg catcgagacc cagagtaaag ccatgacaat gctcaccatt gaacagttat
      481 cctacctgct caagtttgcc attcagaaaa tgaaacagcc agggacagat gcattccaga
      541 agcccgttcc attggaacag caccctgact atgcggaata catcttccat ccaatggacc
      601 tttgtacatt ggaaaagaat gcgaaaaaga aaatgtatgg ctgcacagaa gccttcctgg
      661 ctgatgcaaa gtggattttg cacaactgca tcatttataa tgggggaaat cacaaattga
      721 cgcaaatagc gaaagtagtc atcaaaatct gtgaacatga gatgaatgaa atcgaagtat
      781 gtccagaatg ttatctagct gcttgccaaa aacgagataa ctggttttgt gagccttgta
      841 gcaatccaca tcctttggtc tgggccaaac tgaaggggtt tccattctgg cctgcaaaag
      901 ctctaaggga taaagacggg caggtcgatg cccgattctt tggacaacat gacagggcct
      961 gggttccaat aaataattgc tacctcatgt ctaaagaaat tcctttttct gtgaaaaaga
     1021 cta
//
