LOCUS       sequence-86609-e        1120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1120)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SP100A-c082
FEATURES             Location/Qualifiers
     source          1..1120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             967..984
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgt gcagcagcca tcatcatcat
       61 catcacggca gcggcagcgg ctctgaccag gaggcaaaac cttcaactga ggacttgggg
      121 gataagaagg aaggtgaata tattaaactc aaagtcattg gacaggatag cagtgagatt
      181 cacttcaaag tgaaaatgac aacacatctc aagaaactca aagaatcata ctgtcaaaga
      241 cagggtgttc caatgaattc actcaggttt ctctttgagg gtcagagaat tgctgataat
      301 catactccaa aagaactggg aatggaggaa gaagatgtga ttgaagttta ccaggagcaa
      361 acgggaggtg aaaatagcaa catttgcgaa gtttgcaaca aatggggtcg cctgttttgt
      421 tgcgatacct gcccgcgtag ctttcacgaa cattgccata tcccgagcgt ggaagcgaac
      481 aaaaacccgt ggagctgtat cttctgccgt atcaaaacca ttcaggaacg ttgtccggaa
      541 agccagagcg gccatcagga aagcgaagtg ctgatgcgcc agatgctgcc ggaagaacag
      601 ctgaaatgcg aatttctgct gctgaaagtt tattgcgata gcaaaagctg tttctttgcg
      661 agcgaaccgt attataaccg cgaaggtagc cagggcccgc agaaaccgat gtggctgaac
      721 aaagtgaaaa ccagcctgaa cgaacagatg tatacccgcg tggaaggctt tgttcaggat
      781 atgcgtctga tttttcataa tcataaagaa ttttatcgtg aagataaatt cacccgcctg
      841 ggtattcagg tgcaggatat tttcgaaaaa aactttcgca acatttttgc cattcaggaa
      901 accagcaaat gaagcggtgg cggtctgaac gacatctttc gaggctcaga aatcgaatgg
      961 cacgaacacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct
     1021 gagttggctg ctgccaccgc tgagcatact agcataaccc ttggggcctc taacgggtct
     1081 tgagggtttt tgctgaaagg agaactatat ccggattggc
//
