LOCUS       sequence-86610-e        1107 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1107)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SHKBP1A-c003
FEATURES             Location/Qualifiers
     source          1..1107
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..108
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             486..503
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(556..574)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      570..617
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      654..1105
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(741..760)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     951..972
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat
       61 cattcttctg gtgtagatct gggtaccgag aacctgtact tccaatccat gggggaagtc
      121 atccatctga atgtgggagg caagagattc agtacctctc gccagactct cacctggatc
      181 ccagactcct tcttctccag tcttctgagc ggacgcatct cgacgctgaa agatgagacc
      241 ggagcaatct tcatcgacag ggaccctaca gtcttcgccc ccatcctcaa cttcctgcgc
      301 accaaagagt tggatcccag gggtgtccac ggttccagcc tcctccatga agcccagttc
      361 tatgggctca ctcctctggt tcgtcgcctg cagcttcgag aggagttgga tcgatcttct
      421 tgacagtaaa ggtggatacg gatccgaatt cgagctccgt cgacaagctt gcggccgcac
      481 tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg
      541 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
      601 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat gggacgcgcc
      661 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
      721 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
      781 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
      841 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
      901 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
      961 gttccaaact ggaaaaaaca ctcaacccta tctcggtcta ttcttttgat ttataaggat
     1021 ttgccgattt cggcctattg gttaaaaatg agctgattta acaaaattta acgcgaattt
     1081 taaaaaatat taacgcttac aatttag
//
