LOCUS       sequence-86611-e         579 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA
  TITLE     Multiplex CRISPR/Cas9-based genome engineering from a single 
            lentiviral vector.
  JOURNAL   Nucleic Acids Res. 2014 Aug 13. pii: gku749.
  PUBMED    25122746
REFERENCE   2  (bases 1 to 579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Aug 13. pii: gku749."
COMMENT     Sequence Label: pLV hUbC-dCas9 VP64-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..579
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      141..150
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             150..215
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             222..242
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta
       61 gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag acgaagcttg
      121 ggctgcaggt cgactctaga gccaccatgg actacaaaga ccatgacggt gattataaag
      181 atcatgacat cgattacaag gatgacgatg acaagatggc ccccaagaag aagaggaagg
      241 tgggccgcgg aatggacaag aagtactcca ttgggctcgc catcggcaca aacagcgtcg
      301 gctgggccgt cattacggac gagtacaagg tgccgagcaa aaaattcaaa gttctgggca
      361 ataccgatcg ccacagcata aagaagaacc tcattggcgc cctcctgttc gactccgggg
      421 aaaccgccga agccacgcgg ctcaaaagaa cagcacggcg cagatatacc cgcagaaaga
      481 atcggatctg ctacctgcag gagatcttta gtaatgagat ggctaaggtg gatgactctt
      541 tcttccatag gctggaggag tcctttttgg tggaggagg
//
